LOCUS       Part1                   5886 bp ds-DNA     circular     09-JUN-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1745..2593
                     /label=Part1-1
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          join(5607..5886,1..1790)
                     /label=pSB1C3
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1651..1670
                     /label=VF2 (BBa_G00100)
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1769..1790
                     /label=RFC[10] Prefix
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          4502..5656
                     /label=Part1-4
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5761..5780)
                     /label=VR (BBa_G00101)
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    5607..5627
                     /label=RFC[10] Suffix
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2564..3562
                     /label=Part1-2
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          3533..4531
                     /label=Part1-3
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4315..5463
                     /label=mvaS (Hydroxymethylglutaryl-CoA synthase;
                     Enterococcus faecalis)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1873..4281
                     /label=mvaE (Acetyl-CoA acetyltransferase/HMG-CoA
                     reductase; Enterococcus faecalis)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             1852..1866
                     /label=BBa_B0030
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             4294..4308
                     /label=BBa_B0030(1)
                     /ApEinfo_label="BBa_B0030"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        1791..1845
                     /label=BBa_R0011
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5478..5606
                     /label=BBa_B0015
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
       61 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      181 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      421 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      721 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
      781 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      841 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      901 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      961 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1021 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1081 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     1141 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1201 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1261 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1321 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1381 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1441 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1501 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1561 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1621 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     1681 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     1741 ccttagcttt cgctaaggat gatttctggA attcgcggcc gcttctagag aattgtgagc
     1801 ggataacaat tgacattgtg agcggataac aagatactga gcacatacta gattaaagag
     1861 gagaaatact agATGAAAAC AGTGGTCATT ATTGATGCAT TACGTACGCC GATCGGTAAG
     1921 TACAAAGGGT CACTTTCTCA GGTGTCGGCC GTTGACCTGG GTACGCACGT GACCACTCAG
     1981 CTGCTGAAAC GTCATTCAAC GATCAGTGAA GAAATCGATC AAGTCATTTT CGGTAACGTT
     2041 TTACAGGCAG GGAACGGTCA GAACCCTGCC CGGCAGATTG CGATTAATTC TGGTCTTAGC
     2101 CACGAAATTC CTGCTATGAC TGTGAATGAA GTATGTGGGT CCGGCATGAA AGCAGTGATT
     2161 CTGGCGAAGC AATTGATCCA GCTGGGTGAA GCGGAAGTCC TGATCGCGGG TGGTATCGAA
     2221 AATATGTCCC AGGCGCCAAA ACTCCAACGG TTTAATTATG AGACCGAATC CTACGATGCC
     2281 CCGTTCTCTT CCATGATGTA TGACGGTCTT ACGGATGCTT TTAGCGGCCA GGCTATGGGT
     2341 TTGACCGCCG AAAACGTCGC CGAAAAATAC CATGTGACCC GTGAGGAACA GGACCAATTC
     2401 TCGGTCCACA GCCAACTGAA AGCCGCCCAA GCACAGGCAG AAGGCATTTT CGCGGATGAG
     2461 ATTGCTCCAT TGGAGGTGAG CGGTACTTTA GTCGAGAAAG ATGAAGGAAT CCGTCCAAAT
     2521 AGCTCCGTGG AGAAACTGGG CACCCTGAAA ACCGTTTTCA AAGAAGACGG CACCGTCACG
     2581 GCCGGCAATG CCTCAACGAT TAACGATGGG GCTTCGGCCT TGATTATTGC GTCACAAGAA
     2641 TATGCTGAAG CTCACGGTCT TCCGTATCTG GCTATTATTC GCGATAGCGT AGAAGTGGGT
     2701 ATTGACCCGG CGTATATGGG TATTTCACCG ATCAAAGCCA TTCAGAAACT GCTGGCACGC
     2761 AACCAACTTA CTACAGAAGA AATCGATTTA TATGAGATTA ACGAAGCCTT TGCGGCAACC
     2821 AGCATTGTTG TCCAACGCGA ACTGGCCCTG CCTGAAGAAA AAGTAAATAT CTATGGTGGG
     2881 GGCATTTCTC TGGGCCATGC TATTGGTGCC ACGGGTGCCC GCCTCCTGAC GTCTCTTTCA
     2941 TATCAGCTCA ATCAGAAAGA AAAAAAATAC GGGGTGGCGT CTCTGTGCAT CGGCGGCGGA
     3001 CTGGGGCTTG CGATGTTGCT GGAACGGCCG CAGCAAAAAA AAAATAGCCG TTTCTATCAA
     3061 ATGTCGCCGG AAGAACGTCT GGCTTCCTTA TTGAACGAGG GCCAAATTTC GGCGGATACG
     3121 AAAAAAGAGT TTGAAAACAC CGCCCTTTCC TCGCAAATTG CCAACCATAT GATTGAAAAT
     3181 CAGATTTCCG AAACTGAAGT CCCTATGGGC GTTGGCCTGC ATCTTACCGT TGATGAAACC
     3241 GACTATTTGG TGCCAATGGC TACGGAAGAA CCGTCTGTAA TTGCGGCGCT TTCAAACGGT
     3301 GCGAAAATCG CTCAAGGCTT TAAAACCGTG AATCAGCAGC GCCTGATGCG GGGCCAGATT
     3361 GTTTTTTACG ACGTGGCAGA TGCGGAGTCC CTGATCGATG AGTTGCAAGT ACGCGAAACC
     3421 GAGATTTTTC AGCAGGCCGA ATTGAGCTAC CCCTCCATCG TAAAGCGTGG AGGCGGGCTT
     3481 CGCGACTTAC AGTATCGTGC GTTTGACGAA TCATTTGTTA GCGTGGATTT CCTGGTTGAT
     3541 GTAAAAGATG CAATGGGTGC CAATATTGTG AATGCCATGC TCGAAGGTGT AGCGGAATTA
     3601 TTTCGCGAAT GGTTTGCAGA ACAGAAAATC CTTTTCTCTA TTTTGTCCAA TTATGCGACA
     3661 GAATCAGTGG TGACCATGAA AACTGCGATC CCCGTCAGTC GGCTGTCTAA AGGCAGCAAC
     3721 GGCCGCGAAA TTGCGGAAAA AATCGTCCTG GCAAGCCGGT ATGCGAGTTT AGATCCGTAT
     3781 CGCGCAGTTA CGCACAACAA GGGTATCATG AACGGGATTG AAGCGGTCGT ACTGGCGACC
     3841 GGCAACGATA CCCGTGCTGT CTCGGCCTCA TGTCATGCCT TTGCCGTGAA AGAAGGTCGC
     3901 TATCAGGGGC TGACATCCTG GACCCTTGAC GGCGAACAGC TCATTGGGGA GATTTCCGTT
     3961 CCGCTGGCAC TGGCAACGGT GGGCGGGGCG ACCAAAGTCC TGCCCAAATC ACAGGCTGCT
     4021 GCCGATCTGT TGGCAGTAAC CGATGCCAAA GAGCTGAGCC GTGTTGTGGC CGCGGTGGGC
     4081 CTTGCCCAGA ATCTGGCGGC GTTACGTGCA TTAGTCAGCG AAGGGATCCA GAAGGGCCAC
     4141 ATGGCATTGC AGGCTCGTAG CCTGGCCATG ACTGTTGGCG CGACTGGTAA AGAAGTCGAA
     4201 GCCGTCGCTC AGCAGCTTAA GCGCCAAAAA ACAATGAACC AGGACCGCGC GCTTGCGATT
     4261 CTGAACGACC TGCGGAAACA GTAATAAtac tagattaaag aggagaaata ctagATGACA
     4321 ATCGGAATTG ATAAAATCTC CTTCTTTGTC CCGCCATACT ATATTGACAT GACGGCGCTG
     4381 GCTGAGGCCC GGAATGTTGA TCCTGGTAAA TTTCACATCG GCATCGGCCA AGACCAGATG
     4441 GCAGTGAACC CCATTAGCCA GGATATTGTG ACTTTTGCAG CGAATGCCGC AGAGGCAATT
     4501 CTTACAAAGG AAGACAAAGA AGCAATCGAT ATGGTCATTG TAGGTACTGA GAGTTCGATC
     4561 GATGAAAGCA AGGCCGCAGC GGTGGTGCTG CATCGCCTGA TGGGCATCCA GCCATTTGCC
     4621 CGCAGTTTCG AAATCAAAGA AGCGTGTTAT GGAGCGACGG CCGGTCTGCA ACTGGCGAAG
     4681 AATCATGTAG CACTGCACCC AGACAAAAAA GTTCTGGTGG TTGCAGCGGA TATCGCCAAA
     4741 TACGGCCTGA ATAGCGGTGG TGAACCAACG CAGGGCGCCG GTGCCGTAGC CATGTTAGTA
     4801 GCCTCCGAAC CGCGTATCTT AGCGCTGAAA GAGGACAATG TTATGCTGAC CCAGGATATT
     4861 TACGACTTTT GGCGCCCGAC GGGTCATCCT TATCCTATGG TCGATGGTCC CCTTAGTAAT
     4921 GAGACCTACA TCCAAAGCTT CGCCCAAGTG TGGGACGAAC ATAAAAAGCG CACGGGGTTG
     4981 GATTTTGCCG ACTATGACGC TCTGGCGTTT CACATCCCTT ATACTAAAAT GGGGAAAAAA
     5041 GCACTTTTGG CGAAAATTAG CGATCAGACC GAAGCCGAGC AGGAACGTAT TCTGGCACGT
     5101 TATGAAGAAA GCATCATCTA TAGTCGTCGC GTGGGCAACC TGTATACCGG CTCTCTGTAC
     5161 CTGGGCTTAA TTTCGCTGTT AGAAAACGCC ACAACGTTGA CAGCGGGCAA CCAAATTGGT
     5221 CTGTTCTCCT ATGGCTCTGG CGCCGTGGCG GAATTTTTTA CCGGAGAACT GGTGGCGGGC
     5281 TATCAGAACC ATTTGCAAAA GGAAACCCAC CTGGCCCTGC TGGATAATCG CACGGAATTG
     5341 TCCATTGCAG AGTATGAAGC AATGTTCGCT GAGACCCTCG ATACTGATAT CGATCAGACC
     5401 CTTGAGGATG AACTTAAATA TTCGATTAGC GCTATTAACA ACACAGTTCG TTCCTATCGT
     5461 AATTAATAAt actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     5521 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     5581 ttcgggtggg cctttctgcg tttatatact agtagcggcc gctgcagtcc ggcaaaaaag
     5641 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     5701 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5881 ttccac     
//
