LOCUS       Part2_2                 5522 bp ds-DNA     circular     09-JUN-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(5243..5522,1..1790)
                     /label=pSB1C3
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1740..2638
                     /label=Part2-2-1
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1769..1790
                     /label=RFC[10] Prefix
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1651..1670
                     /label=VF2 (BBa_G00100)
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          4347..5291
                     /label=Part2-2-4
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    5243..5263
                     /label=RFC[10] Suffix
                     /ApEinfo_fwdcolor="#ff4809"
                     /ApEinfo_revcolor="#ff4809"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5397..5416)
                     /label=VR (BBa_G00101)
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          2609..3507
                     /label=Part2-2-2
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          3478..4376
                     /label=Part2-2-3
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1812..2357
                     /label=idi (Isopentenyl-diphosphate Delta-isomerase;
                     Escherichia coli K12)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2391..3446
                     /label=ERG20-2 (Farnesyl pyrophosphate synthase;
                     BBa_K517005/Saccharomyces cerevisiae)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3477..5099
                     /label=TPS03 ((E)-beta-ocimene synthase; Arabidopsis
                     thaliana)
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             1791..1805
                     /label=BBa_B0030
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             3459..3470
                     /label=BBa_B0034
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             2370..2384
                     /label=BBa_B0030(1)
                     /ApEinfo_label="BBa_B0030"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5114..5242
                     /label=BBa_B0015
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
       61 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      181 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      421 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      721 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
      781 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      841 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      901 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      961 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1021 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1081 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     1141 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1201 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1261 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1321 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1381 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1441 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1501 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1561 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1621 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     1681 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     1741 ccttagcttt cgctaaggat gatttctggA attcgcggcc gcttctagag attaaagagg
     1801 agaaatacta gATGCAGACG GAACATGTAA TTCTTCTCAA TGCCCAGGGC GTCCCGACCG
     1861 GCACCCTTGA GAAATATGCG GCGCATACGG CGGACACACG TTTACATCTC GCCTTTTCCT
     1921 CTTGGCTGTT TAACGCGAAA GGCCAGCTCC TGGTAACCCG TCGTGCACTG AGTAAAAAAG
     1981 CGTGGCCGGG TGTTTGGACT AACAGTGTGT GTGGCCATCC GCAACTGGGT GAAAGCAACG
     2041 AGGATGCGGT AATTCGCCGC TGTCGCTACG AATTAGGCGT GGAAATCACT CCACCTGAGT
     2101 CCATTTATCC AGACTTCCGT TATCGTGCGA CCGATCCGTC AGGCATCGTG GAAAACGAAG
     2161 TATGCCCGGT CTTTGCAGCA CGCACTACCA GCGCCCTCCA AATTAACGAC GATGAAGTCA
     2221 TGGACTATCA GTGGTGCGAC CTGGCGGACG TTCTTCACGG TATCGACGCC ACCCCGTGGG
     2281 CGTTCAGCCC CTGGATGGTC ATGCAAGCAA CGAATCGTGA AGCGCGCAAA CGTCTGTCTG
     2341 CGTTCACGCA GCTCAAGTAA TAAtactaga ttaaagagga gaaatactag ATGGCCAGCG
     2401 AAAAAGAAAT TCGGCGTGAA CGTTTTTTAA ACGTCTTCCC CAAACTGGTG GAAGAATTGA
     2461 ATGCGAGTCT GCTGGCTTAC GGCATGCCAA AAGAAGCATG TGATTGGTAC GCACACAGTC
     2521 TGAATTATAA CACCCCAGGA GGAAAGCTTA ACCGCGGCCT CAGCGTGGTC GACACTTACG
     2581 CAATCCTGAG TAATAAAACC GTTGAACAGC TGGGTCAGGA GGAGTACGAA AAAGTAGCGA
     2641 TTCTGGGCTG GTGCATTGAA CTGCTCCAGG CATATTTCTT GGTGGCAGAT GATATGATGG
     2701 ACAAGAGCAT CACGCGCCGT GGTCAACCAT GCTGGTACAA AGTCCCCGAA GTGGGTGAAA
     2761 TCGCAATTAA TGATGCGTTC ATGCTGGAAG CGGCAATTTA TAAGCTCCTG AAAAGCCATT
     2821 TTCGCAACGA GAAGTACTAC ATCGATATCA CAGAGCTGTT TCATGAGGTG ACTTTTCAGA
     2881 CCGAGCTGGG ACAGCTGATG GATTTGATCA CGGCACCGGA AGACAAAGTA GATCTGTCCA
     2941 AATTTTCTCT TAAAAAACAC AGCTTTATCG TAACATTTGA AACTGCATAT TATTCGTTTT
     3001 ATCTGCCGGT TGCGTGGCCA TGCACCTTAC CCGTCTCCCG CGATGAAAAA GATCTTAAAC
     3061 AGGCGCGGGA TGTGCTGATT CCATTAGGGG AATATTTTCA GATCCAAGAT GATTACCTTG
     3121 ACTGTTTCGG AACGCCGGAG CAGATCGGGA AAATTGGCAC CGATATCCAG GATAATAAGT
     3181 GCTCTTGGGT CATCAATAAA GCGCTTGAAT TAGCCAGCGC CGAACAGCGG AAAACGTTAG
     3241 ACGAGAACTA CGGTAAAAAA GACTCTGTTG CGGAAGCCAA ATGTAAAAAA ATCTTCAACG
     3301 ATTTAAAAAT CGAGCAGCTG TATCACGAAT ATGAGGAATC TATCGCCAAA GATTTAAAAG
     3361 CAAAGATTAG CCAGGTGGAT GAAAGCCGGG GGTTCAAAGC AGACGTACTC ACCGCGTTCC
     3421 TTAATAAAGT TTATAAACGT TCGAAATAAT AAtactagaa agaggagaaa tactagATGC
     3481 GCCGCTCCGC CAATTATCAG CCGTCACTGT GGCAGCATGA ATACCTCCTG TCTTTAGGCA
     3541 ACACATATGT TAAAGAAGAC AATGTAGAAC GCGTGACGTT GCTGAAACAG GAGGTTTCCA
     3601 AAATGTTGAA TGAAACGGAA GGCCTGCTCG AGCAGCTGGA ATTAATCGAC ACACTTCAGC
     3661 GCCTGGGGGT AAGTTACCAC TTCGAGCAGG AAATCAAAAA AACTTTAACC AATGTGCATG
     3721 TGAAAAATGT CCGCGCGCAT AAAAATCGCA TCGATCGTAA TCGTTGGGGT GATCTCTACG
     3781 CAACCGCACT GGAGTTCCGC TTACTGCGTC AGCATGGCTT CAGCATTGCA CAGGATGTCT
     3841 TTGATGGTAA TATTGGCGTC GACCTTGACG ATAAAGACAT CAAGGGGATT CTTAGCCTCT
     3901 ACGAAGCATC CTATTTATCG ACACGCATCG ATACGAAACT GAAAGAAAGC ATTTACTATA
     3961 CGACAAAACG TCTCCGTAAA TTTGTGGAAG TCAACAAAAA CGAAACCAAA AGTTATACTC
     4021 TTCGCCGCAT GGTTATTCAT GCCCTGGAAA TGCCGTACCA CCGTCGTGTC GGACGTCTGG
     4081 AAGCGCGTTG GTATATTGAA GTGTATGGCG AGCGGCACGA TATGAACCCG ATTTTACTCG
     4141 AACTCGCCAA ACTGGACTTC AATTTTGTCC AGGCGATTCA TCAGGACGAA CTTAAATCAC
     4201 TGTCAAGTTG GTGGTCAAAA ACAGGTTTAA CGAAACATCT GGATTTTGTT CGCGACCGTA
     4261 TCACGGAGGG ATACTTTTCT AGCGTTGGCG TCATGTATGA ACCTGAATTT GCCTATCACC
     4321 GTCAGATGCT GACCAAAGTC TTTATGTTGA TTACTACAAT CGACGACATT TATGATATTT
     4381 ATGGCACGCT CGAAGAACTC CAACTTTTTA CTACCATTGT AGAGAAGTGG GATGTAAATC
     4441 GTTTAGAGGA ACTTCCGAAC TATATGAAAC TTTGTTTTTT GTGCCTGGTG AATGAAATTA
     4501 ATCAGATTGG CTACTTTGTG TTACGTGATA AAGGCTTCAA CGTAATTCCG TACCTGAAGG
     4561 AATCCTGGGC GGACATGTGT ACAACGTTCC TCAAGGAGGC CAAATGGTAC AAGTCCGGGT
     4621 ATAAACCGAA TTTCGAAGAA TACATGCAGA ATGGGTGGAT TTCTTCATCA GTGCCCACCA
     4681 TTCTGCTGCA TCTGTTTTGC CTTCTGTCGG ATCAAACCTT GGACATTCTG GGCAGTTATA
     4741 ATCATAGCGT GGTCCGCTCT TCAGCAACGA TTCTGCGGTT GGCAAACGAT CTTGCGACCT
     4801 CGTCGGAAGA GTTGGCGCGT GGTGACACAA TGAAGTCTGT GCAGTGCCAT ATGCATGAAA
     4861 CAGGTGCCAG TGAAGCAGAG TCCCGCGCCT ATATTCAAGG TATTATCGGC GTGGCGTGGG
     4921 ATGACCTGAA TATGGAAAAA AAATCCTGCC GCTTGCATCA GGGCTTTCTG GAGGCGGCGG
     4981 CAAACCTTGG CCGTGTTGCT CAGTGTGTGT ACCAGTATGG GGATGGGCAT GGGTGTCCTG
     5041 ACAAGGCCAA AACCGTGAAT CATGTGCGCT CTCTGCTGGT ACACCCGTTG CCGCTGAACT
     5101 AATAAtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     5161 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     5221 ggtgggcctt tctgcgttta tatactagta gcggccgctg cagtccggca aaaaagggca
     5281 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
     5341 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5401 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5461 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5521 ac     
//
