View source for Team:Stony Brook/Notebook/Cancer-W9

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<title> Week 9: 8/22 - 8/28</title>
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<h1> Week 9: 8/22 - 8/28</h1>
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<h2 >8/22 </h2>
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<h4> PCR of new IDT Shipments </h4>
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<h5> AmilCP </h5>
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<th> Contents </th>
<th> ul </th>
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<td> Q5 MasterMix </td>
<td> 12.5 </td>
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<tr>
<td> H<sub>2</sub>O</td>
<td> 9 </td>
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<td> 10 uM F Primer </td>
<td> 1.25 </td>
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<td> 10 uM R Primer </td>
<td> 1.25 </td>
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<td> Template </td>
<td> 1 </td>
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<h5> IDT #1</h5>
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<th> Contents </th>
<th> ul </th>
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<td> Q5 MasterMix </td>
<td> 12.5 </td>
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<td> H<sub>2</sub>O</td>
<td> 6.5</td>
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<td> 10 uM F Primer </td>
<td> 2.5 </td>
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<td> 10 uM R Primer </td>
<td> 2.5 </td>
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<td> Template </td>
<td> 1 </td>
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<h5> IDT #2</h5>
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<th> Contents </th>
<th> ul </th>
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<td> Q5 MasterMix </td>
<td> 12.5 </td>
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<td> H<sub>2</sub>O</td>
<td> 6.5</td>
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<td> 10 uM F Primer </td>
<td> 2.5 </td>
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<td> 10 uM R Primer </td>
<td> 2.5 </td>
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<td> Template </td>
<td> 1 </td>
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<h4> PCR Settings: 25 Cycles
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<th> Phase </th>
<th> Temperature (°C) </th>
<th> Time (sec)</th>
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<td> Initial Denaturation </td>
<td> 98 </td>
<td> 30</td>
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<td> Denaturation </td>
<td> 98 </td>
<td> 10 </td>
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<td> Annealing </td>
<td> 65</td>
<td> 30 </td>
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<td> Extension </td>
<td> 72 </td>
<td> 120 </td>
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<td> Final Extension </td>
<td> 72 </td>
<td> 120 </td>
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<h2> 8/23 </h2>
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<h2> 8/24 </h2>
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