Difference between revisions of "Tracks/Measurement/Interlab study/Flow Cytometry Overview"
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<li>A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser an a 530/30 filter, but the details may vary.</li> | <li>A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser an a 530/30 filter, but the details may vary.</li> | ||
<li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li> | <li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li> | ||
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Revision as of 19:49, 26 April 2016
Measurement with Flow Cytometry
This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.
In order to carry out this measurement protocol, you will need:
- A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser an a 530/30 filter, but the details may vary.
- Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for.
All teams using a flow cytometer to measure GFP must finish the following items to fulfill the InterLab:
- Email your completed Excel file to measurement (AT) igem (DOT) org [note: the empty template file is provided below]
- Fill in the Flow Cytometer Form provided at the bottom of this page
[[Excel File Template]]
Important! If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:
