Revision as of 21:15, 13 September 2016

BioPhotovoltaics

Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.

Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.

Note

Note that parts must be documented on the Registry. This page serves to showcase the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.

Adding parts to the registry

You can add parts to the Registry at our Add a Part to the Registry link.

We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you do need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)

What information do I need to start putting my parts on the Registry?

The information needed to initially create a part on the Registry is:

Part Name
Part type
Creator
Sequence
Short Description (60 characters on what the DNA does)
Long Description (Longer description of what the DNA does)
Design considerations

We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.

Inspiration

We have a created a collection of well documented parts that can help you get started.

You can also take a look at how other teams have documented their parts in their wiki:

Part Table

<groupparts>iGEM2016 Example</groupparts>

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-<h5>Restriction digests – promoter J23119</h5>
-<p>1. Digest 2ug of the DNA. Promoter concentration is 87ng/ul, so we need 23ul of the mini prep.</p>
-<p>2. Add 2.5ul of the 10X buffer and 2ul of enzymes (1ul of each enzyme)</p>
-<p>3. Digest the promoter with Spe and Pst and label it prom dig. </p>
-<p>4. Incubate the restriction digest at 37°C for 2 hours, and then 80°C for 20min to heat kill the enzymes.</p>
-<p>5. Clean the Spe/Pst fragment using the PCR cleanup kit.</p>
-<h5>Restriction digests – K592025, K1172501 and K1172303 and cmpA</h5>
-<p>1. Add 2ug of DNA to be digested – 13.5ul for K592025, 12.6ul for K1172501 and 9ul for K1172303. Adjust each one to 20 ul with distilled H2O. Add 2.5ul of the 10X buffer and 1ul of each enzyme.</p>
-<p>2. Add 01ul of XbaI and 1ul PstI to these preps and label them amil dig, rib dig and opr dig.</p>
-<p>3. Incubate the restriction digest at 37°C for 2 hours, and then 80°C for 20min to heat kill the enzymes. </p>
-<p>4. Run these fragments on the gel and separate them. From the gel, isolate the fragments containing these parts (shorter fragments on the gel) and cmpA should just be purified using the PCR cleanup kit. </p>
-<h5>Restriction digests – J04450, empty vector for the new part and cmpA for submission</h5>
-<p>1. Digest 2ug of the DNA – 17ul of the DNA topped up to 20ul with water.</p>
-<p>2. Add 2.5ul of the 10X buffer and 2ul of enzymes (1ul of each enzyme).</p>
-<p>3. Digest the empty plasmid with Eco and Spe and label it empty dig. </p>
-<p>4. Incubate the restriction digest at 37°C for 2 hours, and then 80°C for 20min to heat kill the enzymes.
-<p>5. Run on a gel and extract the larger fragment. For cmpA – digest it with Eco and Spe and
-cleanup using the PCR cleanup kit.</p>
-<p>Culture received as a streak on a BG11 + glucose plates. Culture immediately resetareked on another plate under sterile conditions and a liquid stock set up.
-Liquid stock was set up using 200ml X1 BG11 solution and 5 loops of the original culture from the streak plate. Liquid culture is set up under 26oC with 2000 lux.</p>
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