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Revision as of 21:36, 14 September 2016
June
Week One
07/06/16
- Made 2 x 400mL 75% EtOH
- Made 30 x 1mL Chloramphenicol (CAM) stocks
- Created in silico primers for regular linear backbone
08/06/16
- Prepared and autoclaved LB broth
- Prepared SOC media
09/06/16
- Autoclaved SOC media and aliquoted 30 x 1mL stocks
- Obtained DH10β cells and made 5mL and 100mL overnight cultures
10/06/16
- Made TSS buffer
- Grew cells to OD600 of 0.34
- Made aliquots of chemically competent cells
- Created small nickel plasmids
Week Two
13/06/16
- LB prep
- Autoclaved 1.5mL microcentrifuge tubes
- Carried out iGEM transformation efficiency protocol
- Plated 17 plates with inoculating loops:
- 3 x 0.5pg/uL
- 3 x 5pg/uL
- 3 x 10pg/uL
