Difference between revisions of "Team:Aix-Marseille/Integrated Practices/Process"
(→Glossary) |
(→Siderophore mediated recovery) |
||
| Line 26: | Line 26: | ||
So where is the innovation in this step? Firstly, in our case, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] won't be applied on the same materials where is commonly used, in our cases not ores but a leachate of ashes. Basically the main difference will be the metal concentration. | So where is the innovation in this step? Firstly, in our case, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] won't be applied on the same materials where is commonly used, in our cases not ores but a leachate of ashes. Basically the main difference will be the metal concentration. | ||
| − | Secondly, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is usually synthesized chemically, we'll rather produce it in high amounts with bacteria. Indeed, [[Team:Aix-Marseille/Description#Overview|operon]] of the Desferrioxamine B biosynthesis will be cloned into a E. coli bacteria strains in order to produce it, hence lowering the costs of required basic matter as production by bacteria needs especially an appropriate medium and good growth conditions. | + | Secondly, [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is usually synthesized chemically, we'll rather produce it in high amounts with bacteria. Indeed, [[Team:Aix-Marseille/Description#Overview|operon]] of the Desferrioxamine B biosynthesis from ''Streptomyces coelicolor''will be cloned into a E. coli bacteria strains in order to produce it, hence lowering the costs of required basic matter as production by bacteria needs especially an appropriate medium and good growth conditions. |
[[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is a derivative of diamines moelcule and therefore its [[Team:Aix-Marseille/Experiments|Biosynthesis]] start with a amino acid, lysine. Lysine is quite expansive, and as we are aware about the cost of our process we decided to use a cheap source of lysine the [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|corn steep liquor]]. Such a lysine source is already in use in industry since it's cheap, amino acid provided, produced in industrial amounts and well known as a excellent source of nitrogen in growth media. So in this step we hope we could produce [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] in high quantities with a affordable cost. | [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] is a derivative of diamines moelcule and therefore its [[Team:Aix-Marseille/Experiments|Biosynthesis]] start with a amino acid, lysine. Lysine is quite expansive, and as we are aware about the cost of our process we decided to use a cheap source of lysine the [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|corn steep liquor]]. Such a lysine source is already in use in industry since it's cheap, amino acid provided, produced in industrial amounts and well known as a excellent source of nitrogen in growth media. So in this step we hope we could produce [[Team:Aix-Marseille/Integrated_Practices/Process#Glossary|DFHOB]] in high quantities with a affordable cost. | ||
