Difference between revisions of "Team:TEC-Costa Rica/Project/Safety"
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<li>Cleaning and Sterilization of the Lab</li> | <li>Cleaning and Sterilization of the Lab</li> | ||
</ul> | </ul> | ||
| + | |||
| + | <div id="services" class="container-fluid text-center"> | ||
| + | <h2>SERVICES</h2> | ||
| + | <h4>What we offer</h4> | ||
| + | <br> | ||
| + | <div class="row slideanim"> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-off logo-small"></span> | ||
| + | <h4>POWER</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-heart logo-small"></span> | ||
| + | <h4>LOVE</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-lock logo-small"></span> | ||
| + | <h4>JOB DONE</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br><br> | ||
| + | <div class="row slideanim"> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-leaf logo-small"></span> | ||
| + | <h4>GREEN</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-certificate logo-small"></span> | ||
| + | <h4>CERTIFIED</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-wrench logo-small"></span> | ||
| + | <h4 style="color:#303030;">HARD WORK</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | <br><br> | ||
| + | <div class="row slideanim"> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-leaf logo-small"></span> | ||
| + | <h4>GREEN</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-certificate logo-small"></span> | ||
| + | <h4>CERTIFIED</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | <div class="col-sm-4"> | ||
| + | <span class="glyphicon glyphicon-wrench logo-small"></span> | ||
| + | <h4 style="color:#303030;">HARD WORK</h4> | ||
| + | <p>Lorem ipsum dolor sit amet..</p> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
<h2>Project safety</h2> | <h2>Project safety</h2> | ||
<p>In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.</p> | <p>In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.</p> | ||
</main> | </main> | ||
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| + | if (pos < winTop + 600) { | ||
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| + | }); | ||
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</body> | </body> | ||
</html> | </html> | ||
{{:Team:TEC-Costa_Rica/Template:Scripts}} | {{:Team:TEC-Costa_Rica/Template:Scripts}} | ||
{{:Team:TEC-Costa_Rica/Template:Footer}} | {{:Team:TEC-Costa_Rica/Template:Footer}} | ||
Revision as of 00:56, 23 September 2016
Safety
Our project is related to the detection of a human disease via a biomarker molecule found in the urine of patients with prostate cancer. Since local regulation does not allow us to work with human samples, we designed our project in a way that it can work as a proof-of-concept of a detection system and pose no risk to human health.
Lab work
During our work in the lab, we followed a series of rules to ascertain that no risks were undertaken.
- Use of lab coat
- Use of nitrile gloves
- Use of safety goggles
- Management of bacteria in biosafety cabinet
- Usage of volatile chemicals in fume hood
- Correct disposal of bio-hazardous residues
- Separate areas for work with bacteria, DNA and electrophoresis
- Cleaning and Sterilization of the Lab
SERVICES
What we offer
Project safety
In order to have a functional system without having to use human samples, we decided to synthesize a DNA fragment of the gene we are going to detect, so we can express it in the bacteria. Then, we are working with dCas9, inteins, TEV protease, and GFP proteins, which are not from pathogenic organisms and were obtained from the Distribution or as gBlocks. All the experiments and cloning is made in Escherichia coli strains DH5alpha, TOP10 and BL21. No pathogenic organisms are used in the lab.
