Difference between revisions of "Team:Paris Bettencourt/Results"

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<h2 class="red">Week 5th September-11th September</h2>
 
<h2 class="red">Week 5th September-11th September</h2>
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<h3>Automated 96 well plate of fabric</h3>
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An automated 96 well microplate for fabric was made using a fluorescent microplate, 96 well tip holder and laser-cut cotton circles.  
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We got an idea of using fluorescent 96 well plate, placing cotton circles inside the wells and gluing the tip holder(which is yellow in the images). This way there is no diffusion, cotton is trapped inside the wells, as the well's inner diameter is bigger than cotton circle diameter of 6.5mm and the circles of tip holder are less than 6.5mm. This is an arrant analog of plastic microplate we tried earlier and the pdms microplate.
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1. Spread flat a wet cotton sheet on glass substrate of 96 well plate dimension.
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2. 96 Wet cotton circles of 6.5mm are laser cut on glass substrate.
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3. Remove the fabric. The circles of cotton stay on the glass substrate.
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4. Press the glass substrate against fluorescent microplate such that all circles align with the wells and move the glass substrate laterally so that all the cotton circles fall into the wells.        
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5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue.
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6. Cover it with a lid and the microplate is ready to use on cotton fabric. The same can be done for other fabrics like silk, Polystyrene, nylon etc.
 
 
 
         
 
         
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<img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ />
 
<img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ />
 
<img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ />
 
<img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ />
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    <h3>Dilution test</h3>
 
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We did a a dilution test to see if we can clearly witness the change in the intensity of the wine of different dilutions in the Microplate. The results are terrific. We could find the difference in intensity of wells from each other and between before dilution test and after dilution test.
 
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<img class="assay" src="https://static.igem.org/mediawiki/2016/7/75/Paris_Bettencourt-Notebook_Assay_Finaldesign_Dilutiontest.jpg" alt=« Success" height=“150px“ />
 
<img class="assay" src="https://static.igem.org/mediawiki/2016/7/75/Paris_Bettencourt-Notebook_Assay_Finaldesign_Dilutiontest.jpg" alt=« Success" height=“150px“ />
 
<img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ />
 
<img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ />

Revision as of 18:46, 30 September 2016



Week 5th September-11th September

An automated 96 well microplate for fabric was made using a fluorescent microplate, 96 well tip holder and laser-cut cotton circles. 1. Spread flat a wet cotton sheet on glass substrate of 96 well plate dimension.
2. 96 Wet cotton circles of 6.5mm are laser cut on glass substrate.
3. Remove the fabric. The circles of cotton stay on the glass substrate.
4. Press the glass substrate against fluorescent microplate such that all circles align with the wells and move the glass substrate laterally so that all the cotton circles fall into the wells.
5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue.
6. Cover it with a lid and the microplate is ready to use on cotton fabric. The same can be done for other fabrics like silk, Polystyrene, nylon etc.

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Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org