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<p><h3><B> June 20, 2016:</B></h3></p> | <p><h3><B> June 20, 2016:</B></h3></p> | ||
<p> | <p> | ||
− | <a href="#exp1"><h4> Dosage of pET43.1</h4></a></br> | + | <a href="#exp1"><h4> 16. Dosage of pET43.1</h4></a></br> |
− | <a href="#exp2"><h4> Checkin of stab cultures</h4></a></br> | + | <a href="#exp2"><h4> 17. Checkin of stab cultures</h4></a></br> |
− | <a href="#exp3"><h4> Transformation of pET43.1a (+)</h4></a></br> | + | <a href="#exp3"><h4> 18. Transformation of pET43.1a (+)</h4></a></br> |
</p> | </p> | ||
<p><h3><B> June 21, 2016:</B></h3></p> | <p><h3><B> June 21, 2016:</B></h3></p> | ||
<p> | <p> | ||
− | <a href="#exp4"><h4> Analyse </h4></a></br> | + | <a href="#exp4"><h4> 19. Analyse </h4></a></br> |
− | <a href="#exp5"><h4> Dosage of pET43.1a(+) </h4></a></br> | + | <a href="#exp5"><h4> 20. Dosage of pET43.1a(+) </h4></a></br> |
− | <a href="#exp6"><h4> Digestion of pET43.1 and C1 and C2</h4></a></br> | + | <a href="#exp6"><h4> 21. Digestion of pET43.1 and C1 and C2</h4></a></br> |
− | <a href="#exp7"><h4> Electrophoresis of pET43.1a(+) and the inserts C1 and C2:</h4></a></br> | + | <a href="#exp7"><h4> 22. Electrophoresis of pET43.1a(+) and the inserts C1 and C2:</h4></a></br> |
− | <a href="#exp8"><h4> Gel extraction:</h4></a></br> | + | <a href="#exp8"><h4> 23. Gel extraction:</h4></a></br> |
− | <a href="#exp9"><h4> Dephosphorylation of pET43.1a(+):</h4></a></br> | + | <a href="#exp9"><h4> 24. Dephosphorylation of pET43.1a(+):</h4></a></br> |
</p> | </p> | ||
<p><h3><B> June 22, 2016:</B></h3></p> | <p><h3><B> June 22, 2016:</B></h3></p> | ||
<p> | <p> | ||
− | <a href="#exp10"><h4> Ligation of pET43.1a(+) with C1 and C2 </h4></a></br> | + | <a href="#exp10"><h4> 25. Ligation of pET43.1a(+) with C1 and C2 </h4></a></br> |
− | <a href="#exp11"><h4> Transformation of pET43.1a(+) linked with C1 and C2</h4></a></br> | + | <a href="#exp11"><h4> 26. Transformation of pET43.1a(+) linked with C1 and C2</h4></a></br> |
</p> | </p> | ||
<p><B><h3> June 23, 2016:</B></h3></p> | <p><B><h3> June 23, 2016:</B></h3></p> | ||
<p> | <p> | ||
− | <a href="#exp12"><h4> Dosage of the uncut inserts</h4></a></br> | + | <a href="#exp12"><h4> 27. Dosage of the uncut inserts</h4></a></br> |
</p> | </p> | ||