Difference between revisions of "Team:SCAU-China/Safety"

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{{SCAU-China}}
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<link href="https://2016.igem.org/Team:SCAU-China/CSS/result?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<div id="page">
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  <div id="header">
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  <div class="logo">
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/b/b2/T--SCAU-China--logo_2.png"></a>
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/e/e3/T--SCAU-China--logo_3.png"></a></div>
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<div class="menu">
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  <ul>
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<li><a href="#">Home</a></li>
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<li><a href="#">Team</a></li>
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<li><a href="#">Project</a></li>
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<li><a href="#">Result</a></li>
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<li><a href="#">Notebook</a></li>
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<li><a href="#">HP</a></li>
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<li><a href="#">Judging</a></li>
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</ul>
 
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  <div class="triangle"><a class="click"></a></div>
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<div class="column full_size">
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  <div id="content">
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<h5>Safe Project Design</h5>
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    <div class="vecter">
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      <ul class="nav">
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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      <a id="vecter"></a>
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      <li><a href="#vecter" class="on">Vecter Constraction</a></li>
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<ul>
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      <li><a href="#tissue">Tissue Culture</a></li>
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<li>Choosing a non-pathogenic chassis</li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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      <li><a href="#hplc">HPLC</a></li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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      <li><a href="#modeling">Modeling</a></li>
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</ul>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/3/31/T--SCAU-China--img_1.jpg"></div>
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      <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
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Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
 
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<div class="column half_size">
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<h5>Safe Lab Work</h5>
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    <div class="vecter">
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      <ul class="nav">
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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      <a id="tissue"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue" class="on">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <p><img src="https://static.igem.org/mediawiki/2016/6/61/T--SCAU-China--img_2.jpg"></p>
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      <p><img src="https://static.igem.org/mediawiki/2016/4/4c/T--SCAU-China--img_3.jpg"></p>
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      <p><img src="https://static.igem.org/mediawiki/2016/7/75/T--SCAU-China--img_4.jpg"></p>
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="pcr"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr" class="on">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <p><img src="https://static.igem.org/mediawiki/2016/0/0f/T--SCAU-China--img_5.jpg"></p>
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      <p><img src="https://static.igem.org/mediawiki/2016/b/b3/T--SCAU-China--img_6.jpg"></p>
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="phenotype"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype" class="on">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <p><img src="https://static.igem.org/mediawiki/2016/9/94/T--SCAU-China--image014.jpg">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/0/02/T--SCAU-China--image015.png">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/f/f6/T--SCAU-China--image016.png"></p><br>
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      <p><img src="https://static.igem.org/mediawiki/2016/8/8a/T--SCAU-China--image020.jpg">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/8/88/T--SCAU-China--image022.png">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/9/93/T--SCAU-China--image018.png"></p>
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="hplc"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc" class="on">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://2016.igem.org/File:T--SCAU-China--image024.png"></div>
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      <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
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HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
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Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div> 
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/0/0b/T--SCAU-China--image026.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/4/42/T--SCAU-China--image028.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/8/84/T--SCAU-China--image030.png"></div>   
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="modeling"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling" class="on">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/2/2e/T--SCAU-China--image032.png"></div>
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      <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 07:39, 5 October 2016

Fig.1 Not I digestion of constructs cotaining different numbers of genes. Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.

    


    

Fig.n HPLC analysis diagram of standard samples of astaxanthin(a); HPLC analysis diagram of samples extract from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);

Compared with the standard samples with HPLC analysis diagram, you can see the sample extract from rice and standard samples have the same peak at the same time, show that the transgenic rice seed which carry the four genes expressed astaxanthin. Results indicate that we are successful synthesize astaxanthin by rice endosperm synthetic .
Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .