Difference between revisions of "Team:Danci-K8/Notebook"

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</table>
 
</table>
  
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<h5>19/7/2016</h5>
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<p>In the lab today: Avital, Gal, Lidor<br>
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Planning 5 gRNA for CRISPR reaction
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</p>
  
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<table>
  
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  <tr>
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    <th>Taste/NoTaste</th>
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    <th>Spacer</th>
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    <th>PAM</th>
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  </tr>
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  <tr>
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    <td>No Taste</td>
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    <td>TCAGTTTTGTGGCAGATGAC</td>
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    <td>AGG</td>
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  </tr>
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  <tr>
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    <td>No Taste</td>
 +
    <td>CACATCAGTGAGGAGATTCT</td>
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    <td>AGG</td>
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  </tr>
 +
  <tr>
 +
    <td>No Taste</td>
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    <td>TCGTGTGTGTCATGAAAGCT</td>
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    <td>TGG</td>
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  </tr>
 +
  <tr>
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    <td>No Taste</td>
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    <td>GGCCACGGGCACGGTTGTAGC</td>
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    <td>CGG</td>
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  </tr>
 +
  <tr>
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    <td> (non targeting)</td>
 +
    <td>aggatcacct gagtccaggg</td>
 +
    <td>-</td>
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  </tr>
  
−
 
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</table>
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Revision as of 14:14, 5 October 2016

Danci-K8

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15/7/2016

In the lab today: Itay, May, Tomer
Planning synthetic DNA
Planning 8 synthetic SNPs and placing an order.

16/7/2016

In the lab today: Itay, May, Tomer
Planning synthetic DNA
Planning 7 synthetic SNPs and placing an order.

The final SNPs (Single nucleotide polymorphism)
Final Order Table

Number Taste RS
1 Sweet rs35874116
2 Sweet rs307355
3 PTC rs713598
4 PTC rs1726866
5 PTC rs1024639
6 Consuming Alcohol rs846672
7 Coffee Bitterness RS765007
8 Coffee Bitterness RS2234001
9 Coffee Bitterness RS2234012
10 Coffee Bitterness RS2227264
11 Grapefruit juice bitterness rs10772420
12 Cilantro Preference rs72921001
13 Taste of NaC1 & Sour rs17492553
14 Taste of NaC1 & Sour rs34160967
16 Umami& Monosodium gluten rs76755863
19/7/2016

In the lab today: Avital, Gal, Lidor
Planning 5 gRNA for CRISPR reaction

Taste/NoTaste Spacer PAM
No Taste TCAGTTTTGTGGCAGATGAC AGG
No Taste CACATCAGTGAGGAGATTCT AGG
No Taste TCGTGTGTGTCATGAAAGCT TGG
No Taste GGCCACGGGCACGGTTGTAGC CGG
(non targeting) aggatcacct gagtccaggg -
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