Line 173: | Line 173: | ||
<p>(non targeting)</p> | <p>(non targeting)</p> | ||
+ | <h5>1/8/2016</h5> | ||
+ | <p>In the lab today: Daniel, Yuval, Shir, Shahar, Amit S., Gal, Amit K.<br> | ||
+ | Collecting DNA samples from 43 people. | ||
+ | </p> | ||
+ | <p>The collecting was done in the framework of school. Each of the participants came to the station we set up at the school and filled out an questionnaire and then answered a questionnaire while tasting. The questionnaire included questions tailored to the tastes of the participants: sweet, umami, sour, salty and bitter. Each participant stated his sensitivity to different tastes when tried, the listing the participant’s phenotype. All data was collected into a general survey which summarized the results of the participants. </p> | ||
+ | <img class="imageCenter" style="width:248px; height:480px; padding:10px;" src="https://static.igem.org/mediawiki/2016/thumb/0/04/K8_dna_sample1.jpg/309px-K8_dna_sample1.jpg"> | ||
+ | <p class="imageText">Shelly has no sensitivity to - PTC</p> | ||
+ | |||
+ | |||
+ | <img class="imageCenter" style="width:337px; height:599px; padding:10px;" src="https://static.igem.org/mediawiki/2016/thumb/d/dd/K8_dna_sample2.jpg/337px-K8_dna_sample2.jpg"> | ||
+ | <p class="imageText">Lior on the other hand cannot stand the taste of PTC</p> | ||
Revision as of 14:31, 5 October 2016
15/7/2016
In the lab today: Itay, May, Tomer
Planning synthetic DNA
Planning 8 synthetic SNPs and placing an order.
16/7/2016
In the lab today: Itay, May, Tomer
Planning synthetic DNA
Planning 7 synthetic SNPs and placing an order.
The final SNPs (Single nucleotide polymorphism)
Final Order Table
Number | Taste | RS |
---|---|---|
1 | Sweet | rs35874116 |
2 | Sweet | rs307355 |
3 | PTC | rs713598 |
4 | PTC | rs1726866 |
5 | PTC | rs1024639 |
6 | Consuming Alcohol | rs846672 |
7 | Coffee Bitterness | RS765007 |
8 | Coffee Bitterness | RS2234001 |
9 | Coffee Bitterness | RS2234012 |
10 | Coffee Bitterness | RS2227264 |
11 | Grapefruit juice bitterness | rs10772420 |
12 | Cilantro Preference | rs72921001 |
13 | Taste of NaC1 & Sour | rs17492553 |
14 | Taste of NaC1 & Sour | rs34160967 |
16 | Umami& Monosodium gluten | rs76755863 |
19/7/2016
In the lab today: Avital, Gal, Lidor
Planning 5 gRNA for CRISPR reaction
Taste/NoTaste | Spacer | PAM |
---|---|---|
No Taste | TCAGTTTTGTGGCAGATGAC | AGG |
No Taste | CACATCAGTGAGGAGATTCT | AGG |
No Taste | TCGTGTGTGTCATGAAAGCT | TGG |
No Taste | GGCCACGGGCACGGTTGTAGC | CGG |
(non targeting) | aggatcacct gagtccaggg | - |
1. TAS2R3 RS765007
CGACAGACTAAATTGGGCAGAGACAAGAGACAGGTACAGTGAAGCAACAGGTAGAGGAGTAGATTAACGAG
AAAAATATCACATCAGTGAGGAGATTCTA[T/G]GTATCAACAGAAAGAACAAAGATCAGGGCTGCCTAATTGC
TGACATGATGGGACTCACCGAGGGGGTGTTCCTGATTCTGTCTGGCACTCAGTTCACACT
2. TAS2R3 RS765007
CGACAGACTAAATTGGGCAGAGACAAGAGACAGGTACAGTGAAGCAACAGGTAGAGGAGTAGATTAACGAG
AAAAATATCACATCAGTGAGGAGATTCTA[T/G]GTATCAACAGAAAGAACAAAGATCAGGGCTGCCTAATTGC
TGACATGATGGGACTCACCGAGGGGGTGTTCCTGATTCTGTCTGGCACTCAGTTCACACT
3. TAS1R1 rs34160967
AGAAGCCTATGCCCGGGCAGACAAGAAGGCCCCTAGGCCTTGCCACAAGGGCTCCTGGTGCAGCAGCAATCA
GCTCTGCAGAGAATGCCAAGCTTTCATG[G/A]CACACACGATGCCCAAGCTCAAAGCCTTCTCCATGAGTTCTG
CCTACAACGCATACCGGGCTGTGTATGCGGTGGCCCATGGCCTCCACCAGCTCCTGGG
4. TAS1R3 rs307377
CACACGCTCCTGGGTCAGCTTCGGCCTAGCGCACGCCACCAATGCCACGCTGGCCTTTCTCTGCTTCCTGGGC
ACTTTCCTGGTGCGGAGCCAGCCGGGC[T/C]GCTACAACCGTGCCCGTGGCCTCACCTTTGCCATGCTGGCCTAC
TTCATCACCTGGGTCTCCTTTGTGCCCCTCCTGGCCAATGTGCAGGTGGTCCTCAG
5. NEGATIVE CONTROL CLASSIC gRNA
(non targeting)
1/8/2016
In the lab today: Daniel, Yuval, Shir, Shahar, Amit S., Gal, Amit K.
Collecting DNA samples from 43 people.
The collecting was done in the framework of school. Each of the participants came to the station we set up at the school and filled out an questionnaire and then answered a questionnaire while tasting. The questionnaire included questions tailored to the tastes of the participants: sweet, umami, sour, salty and bitter. Each participant stated his sensitivity to different tastes when tried, the listing the participant’s phenotype. All data was collected into a general survey which summarized the results of the participants.
Shelly has no sensitivity to - PTC
Lior on the other hand cannot stand the taste of PTC
What should this page have?
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