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<p style="font-size:11pt"> The iGEM Paris-Saclay project is part of the Foundational Advance track, and aims to study the effects of DNA topology on gene expression in E.coli. The purpose is to answer to this question: ''Does bringing a strong promoter closer to a weak promoter influence the expression level of genes located downstream?'' | <p style="font-size:11pt"> The iGEM Paris-Saclay project is part of the Foundational Advance track, and aims to study the effects of DNA topology on gene expression in E.coli. The purpose is to answer to this question: ''Does bringing a strong promoter closer to a weak promoter influence the expression level of genes located downstream?'' | ||
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− | We have designed a new tool based on CRISPR/Cas9 system to bring two specific DNA regions closer. This system is composed of two different | + | We have designed a new tool based on CRISPR/Cas9 system to bring two specific DNA regions closer. This system is composed of two different dCas9s fused with each part of FRB / FKBP12 dimerization system. Each dCas9 will target a specific DNA sequence, one on the chromosome and one on a plasmid, whereas dimerization system will promote the joining of the two dCas9s when rapalog is added. In order to assess whether or not this system works, we have also designed a new tool to visualize the interaction between both dCas9s. This tool is composed of a split GFP attached to two dCas9s. These two small GFP tags will interact with the complementary GFP detector only if the two dCas9s are close enough to interact. <br><br> |
To learn more about our goal:</p> | To learn more about our goal:</p> | ||
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Revision as of 16:46, 7 October 2016