Difference between revisions of "Team:Danci-K8/Proof"

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<h5>Stage one</h5>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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<li>We made sure to create a relatively varied testing system with the number alleles for each of the five tastes that were being checked.</li>
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<li>The people we checked filled out a questionnaire while doing the phenotype test, (characterization of the ability to recognize and feel tastes in practice). The questionnaire was analyzed statistically from the test results.</li>
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<li>Whenever we found a match between the SNP and the sequence tested, the DNA polymerase continued the process of reproduction; the probe was freed and formed a fluorescent signal. We compared the results to the genotype test for all tastes, and for each taste with a number of SNP’s.</li>
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<li>We engineered the SNP to be used for negative monitoring.</li>
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<li>Finally we found a match between genotype phenotype which collected in the study.</li>
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<h5>Stage two</h5>
  
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<li>The “Flavoff” system is derived from a number of steps that depend on each other. The occurrence in the last stage, meaning, cutting the suitable SNP and obtaining the expected modifications, prove the venture feasibility of "Flavoff ". Various combinations of gRNA would be expressed and DNA run on a gel to check for expected band sizes. A comprehensive results analysis will further substantiate the feasibility estimate</li>
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<li>Running negative control A – using the fifth gRNA molecule which is not supposed to identify the encoded SNP for the specific different taste receptors. There is no expected cut by Cas9 in this monitor.</li>
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<li>Running negative control B – using the vector including a negative construct which does not encode any significant SNP. There is no expected cut by Cas9 in this control.</li>
  
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
 
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<h4> What should we do for our proof of concept? </h4>
 
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
 
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<img src="https://static.igem.org/mediawiki/2016/3/38/K8_proofC.png" style="width:800px; height:318px;" class="imageCenterNB">
  
  

Latest revision as of 07:36, 8 October 2016

Danci-K8

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Stage one
  1. We made sure to create a relatively varied testing system with the number alleles for each of the five tastes that were being checked.
  2. The people we checked filled out a questionnaire while doing the phenotype test, (characterization of the ability to recognize and feel tastes in practice). The questionnaire was analyzed statistically from the test results.
  3. Whenever we found a match between the SNP and the sequence tested, the DNA polymerase continued the process of reproduction; the probe was freed and formed a fluorescent signal. We compared the results to the genotype test for all tastes, and for each taste with a number of SNP’s.
  4. We engineered the SNP to be used for negative monitoring.
  5. Finally we found a match between genotype phenotype which collected in the study.
Stage two
  1. The “Flavoff” system is derived from a number of steps that depend on each other. The occurrence in the last stage, meaning, cutting the suitable SNP and obtaining the expected modifications, prove the venture feasibility of "Flavoff ". Various combinations of gRNA would be expressed and DNA run on a gel to check for expected band sizes. A comprehensive results analysis will further substantiate the feasibility estimate
  2. Running negative control A – using the fifth gRNA molecule which is not supposed to identify the encoded SNP for the specific different taste receptors. There is no expected cut by Cas9 in this monitor.
  3. Running negative control B – using the vector including a negative construct which does not encode any significant SNP. There is no expected cut by Cas9 in this control.