Difference between revisions of "Team:IISc Bangalore/Team"

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      <h1>Bioreactor design</h1>
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      <h1>Abhijeet Krishna</h1>
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      <p class="whitef">We realized that manufacturing units use expensive and energy expensive machinery <a href="#refer">[3]</a>. Hence, we engineered our bacteria in such a way that it could do most of the work done by these monstrous machines so that we no longer have to buy them and pay their bills.
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To increase the yield of the product, we came up with the idea of separating cell growth and target protein production so that when the cells have grown up to a high enough cell density, they shuttle all their energy towards the required protein production<a href="#refer"> [4]</a>. Based on the above ideas, we came up with the design of our bioreactor.
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<p>We realized that we would not be able to work on the whole idea this summer so, we focussed on two modules of our design: the Protein production module and the auto-aggregation module leaving the arresting of cell growth for future iGEM teams as a seed for their ideas.
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      <p class="whitef">Year: 3rd,  
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Major:Physics<br>
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"Hello! I have always believed that simple scientific ideas can influence society greatly and that is exactly what I am trying to show in iGEM. I am married to Physics and have an affair with Biology. I am a Calvin and Hobbes fan and can’t resist kebabs."
 
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      <h1>Protein production</h1>
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      <h1>Prabaha Gangopadhyay</h1>
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      <p class="whitef">Induction synthesis of protein of interest is another important step. Microorganisms in bioreactors are induced at the OD, at which the protein production is maximum. <a href="#refer">[5]</a> Because of this, factories install sophisticated machinery to keep track of the OD value of the culture so as to know when to induce the production. We wanted our bacteria to know when to start protein production and start it by itself. Hence, we used the lux quorum sensing system to sense this cell density. <a href="#refer">[6]</a> We have expressed the gene for our protein of interest under the luxI gene so that protein of interest is produced in significant quantities only when the quorum is achieved.(Figure 2) We also wish to tune the quorum sensing system by tweaking with the strength of the promoters involved in quorum sensing.
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      <p class="whitef">Year: 3rd,
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Major: Biology<br>
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"I like learning about mechanisms underlying the existence of life. I find myself comfortable in the overlap of theoretical and experimental biology, because of the extreme interdisciplinary nature of the area, and being at IISc has allowed me to explore it. I am interested in doing my final year project, and eventually my PhD, in Neuroscience. Other than science, I love literature, classical music, and, like any other undergrad, food!"
 
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      <h1>Shreyas Gopalakrishnan</h1>
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      <p class="whitef">To achieve cellular aggregation, we use the famous auto-transporter protein, Antigen43 (Ag43). [7] It has shown to cause auto-aggregation of cells when overexpressed. (Figure 3) We plan to exploit this property of Ag43 to substitute centrifugation. We acknowledge previous iGEM teams who have worked on Ag43 (Hokkaido University, 2012; Aberdeen Scotland, 2014 and some more) as we got the BioBricks for Ag43 readily available. We intend to express Ag43 only when the cells have made the product of interest. Hence, we use the diauxic shift in the media to act as a switch for the expression of Ag43.
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      <p class="whitef">Year: 3rd,  
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Major: Biology; Minor: Physics<br>
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"I am interested in the problems in biology, but using a variety of physical and other methods/approaches to show consistent, robust and reproducible results. Recently, I also became interested in designing experiments, especially in stem cell biology and cell imaging. I'd like to learn general principles from diverse areas of science so that I can apply them in biology. In my free time, I listen to generic pop music."
 
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      <h1>Aiswarya Prasad</h1>
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      <p class="whitef">Year: 2nd,
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Major:Undecided (Biology, eventually)<br>
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"I have always been most fascinated by how life works, by virtue of nothing more than 'molecules'. I enjoy learning and thinking about most things that even remotely belong in the realm of science. I wish to pursue whatever it is that intrigues me most, wherever that takes me. I also enjoy reading, classical music and occasionally gaming."
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      <h1>Ayan Das</h1>
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      <p class="whitef">Year: 2nd,
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Major: Unassigned. (Most likely Biology) <br>
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"I am mostly interested in biology but with a strong inclination towards maths and computation. Papers that don't have equations bore the life out of me! I easily get interested in just about anything. I have no concrete plans for the future. At the moment, I'm just enjoying everything that I am getting to learn. Apart from science, I like writing and playing the keyboard (which I have just started learning). Hopefully, I'll contribute something significant to the scientific community some day." 
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"I am interested in the problems in biology, but using a variety of physical and other methods/approaches to show consistent, robust and reproducible results. Recently, I also became interested in designing experiments, especially in stem cell biology and cell imaging. I'd like to learn general principles from diverse areas of science so that I can apply them in biology. In my free time, I listen to generic pop music."
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      <p class="whitef">Year: 2nd,
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Major: Most likely biology. <br>
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"I am interested in the problems in biology, but using a variety of physical and other methods/approaches to show consistent, robust and reproducible results. Recently, I also became interested in designing experiments, especially in stem cell biology and cell imaging. I'd like to learn general principles from diverse areas of science so that I can apply them in biology. In my free time, I listen to generic pop music."
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Revision as of 19:02, 8 October 2016



Team IISc iGem

nickname

A. Chakraborty

nickname

A. Krishna

nickname

P. Gangopadhyay

nickname

S. Gopalakrishnan

nickname

A. Prasad

nickname

A. Das

nickname

A. Baburaj

nickname

J. Bodosa

More about the team

Other team aspects.
×

Arunavo Chakraborty

Arunavoiisc.jpg

Year: 3rd, Major: Chemistry; Minor: Biology
"I am a chemistry enthusiast with a recent inclination toward biology. What excites me the most about field is the incredible plethora of chemical reactions in biological systems that would (and do) give any chemist a run for their money. Currently, the topics that interest me are enzyme mechanics, peptide design and biomimetic chemistry. My (only) other companions are select manga and MMO (note, not RP) games."
Read more

×

Abhijeet Krishna

Abhijeetiisc.jpg

Year: 3rd, Major:Physics
"Hello! I have always believed that simple scientific ideas can influence society greatly and that is exactly what I am trying to show in iGEM. I am married to Physics and have an affair with Biology. I am a Calvin and Hobbes fan and can’t resist kebabs."
Read more

×

Prabaha Gangopadhyay

Prabahaiisc.jpg

Year: 3rd, Major: Biology
"I like learning about mechanisms underlying the existence of life. I find myself comfortable in the overlap of theoretical and experimental biology, because of the extreme interdisciplinary nature of the area, and being at IISc has allowed me to explore it. I am interested in doing my final year project, and eventually my PhD, in Neuroscience. Other than science, I love literature, classical music, and, like any other undergrad, food!"
Read more

×

Shreyas Gopalakrishnan

Prabahaiisc.jpg

Year: 3rd, Major: Biology; Minor: Physics
"I am interested in the problems in biology, but using a variety of physical and other methods/approaches to show consistent, robust and reproducible results. Recently, I also became interested in designing experiments, especially in stem cell biology and cell imaging. I'd like to learn general principles from diverse areas of science so that I can apply them in biology. In my free time, I listen to generic pop music."
Read more

×

Aiswarya Prasad

Aiswaryaiisc.jpg

Year: 2nd, Major:Undecided (Biology, eventually)
"I have always been most fascinated by how life works, by virtue of nothing more than 'molecules'. I enjoy learning and thinking about most things that even remotely belong in the realm of science. I wish to pursue whatever it is that intrigues me most, wherever that takes me. I also enjoy reading, classical music and occasionally gaming."
Read more

×

Ayan Das

Ayaniisc.jpg

Year: 2nd, Major: Unassigned. (Most likely Biology)
"I am mostly interested in biology but with a strong inclination towards maths and computation. Papers that don't have equations bore the life out of me! I easily get interested in just about anything. I have no concrete plans for the future. At the moment, I'm just enjoying everything that I am getting to learn. Apart from science, I like writing and playing the keyboard (which I have just started learning). Hopefully, I'll contribute something significant to the scientific community some day."
Read more

×

Aneesh Baburaj

Prabahaiisc.jpg

Year: 2nd, Major:
"I am interested in the problems in biology, but using a variety of physical and other methods/approaches to show consistent, robust and reproducible results. Recently, I also became interested in designing experiments, especially in stem cell biology and cell imaging. I'd like to learn general principles from diverse areas of science so that I can apply them in biology. In my free time, I listen to generic pop music."
Read more

×

Jessica

Prabahaiisc.jpg

Year: 2nd, Major: Most likely biology.
"I am interested in the problems in biology, but using a variety of physical and other methods/approaches to show consistent, robust and reproducible results. Recently, I also became interested in designing experiments, especially in stem cell biology and cell imaging. I'd like to learn general principles from diverse areas of science so that I can apply them in biology. In my free time, I listen to generic pop music."
Read more

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