Week 1: May 23 - May 27

Wet Lab

• Training begins
• Grow Top 10 competent cells.

Dry Lab

• Brainstorm genetic circuits for a thallium sensor
• Lab safety training

Week 1 Notebook Back to Top

Week 2: May 31 - June 3

Wet Lab

• Test efficiency of competent cells
• Test cell free extract reaction with T7-GFP plasmid

Dry Lab

• Contact museums and summer programs for outreach opportunities
• Lab safety training

Week 2 Notebook Back to Top

Week 3: June 6 - June 12

Wet Lab

• Transform T7 promoter, amilCP, and terminator
• Begin assembly by ligating linearized T7 promoter and amilCP

Dry Lab

• Contact museums and summer programs for outreach opportunities

Week 3 Notebook Back to Top

Week 4: June 13 - June 17

Wet Lab

• Transform T7-GFP plasmid, lacZ alpha fragment, and eGFP
• Send promising T7 promoter -- amilCP ligations to be sequenced
• Perform double digest of T7 promoter and terminator from last week
• Ligate double-digested T7 promoter to new reporters (lacZ and eGFP)

Dry Lab

• TECBio, DiSCoBio, and Tissue Engineering Camp outreach opportunities set

Week 4 Notebook Back to Top