Difference between revisions of "Team:Edinburgh UG/Safety"

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                 <h2>Demo Box #2</h2>
 
                 <h2>Demo Box #2</h2>
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                 <p>Write something.</p>
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                 <p>Even if one were to insert a BabbleBlock into a cell to amplify, we took precautions so that it will not affect the cell’s function. To that end, we inserted stop codons in all three reading frames of our BabbleBricks, and in both directions, so that if our artificially constructed DNA were to somehow be transcribed, this will not result in nonsensical peptide chains that might interfere with normal cellular processes. Nonsense polypeptides are not well-tolerated in cells and so there are innate cellular mechanisms that eliminate mRNA with premature stop codons in it (Brogna and Wen, 2009). </p>
 
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                <h2>Demo Box #1</h2>
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                <p>Our project has an advantage from a safety perspective in that it is largely cell-free. The process of BabbleBrick assembly only involves the magnetic beads, enzymes and, of course, the Bricks themselves. Our constructs can then be inserted into plasmids and stored in test tubes, and will actually be more stable outside of cells rather than inside them due to spontaneous mutations and replication errors that occur within cells (Allentoft et al., 2012; Lee et al., 2012).
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                <img class="img-circle img-responsive img-center" src="http://placehold.it/300x300" alt="">
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                <h2>Demo Box #2</h2>
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                <p>Even if one were to insert a BabbleBlock into a cell to amplify, we took precautions so that it will not affect the cell’s function. To that end, we inserted stop codons in all three reading frames of our BabbleBricks, and in both directions, so that if our artificially constructed DNA were to somehow be transcribed, this will not result in nonsensical peptide chains that might interfere with normal cellular processes. Nonsense polypeptides are not well-tolerated in cells and so there are innate cellular mechanisms that eliminate mRNA with premature stop codons in it (Brogna and Wen, 2009). </p>
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            <div class="col-sm-4">
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                <img class="img-circle img-responsive img-center" src="http://placehold.it/300x300" alt="">
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                <h2>Demo Box #3</h2>
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                <p>Write something.</p>
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            </div>
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Revision as of 17:55, 9 October 2016

Safety



Safety

Demo 1

Description 1

Description 11

Button 1 »

Demo Box #1

Our project has an advantage from a safety perspective in that it is largely cell-free. The process of BabbleBrick assembly only involves the magnetic beads, enzymes and, of course, the Bricks themselves. Our constructs can then be inserted into plasmids and stored in test tubes, and will actually be more stable outside of cells rather than inside them due to spontaneous mutations and replication errors that occur within cells (Allentoft et al., 2012; Lee et al., 2012).

Demo Box #2

Even if one were to insert a BabbleBlock into a cell to amplify, we took precautions so that it will not affect the cell’s function. To that end, we inserted stop codons in all three reading frames of our BabbleBricks, and in both directions, so that if our artificially constructed DNA were to somehow be transcribed, this will not result in nonsensical peptide chains that might interfere with normal cellular processes. Nonsense polypeptides are not well-tolerated in cells and so there are innate cellular mechanisms that eliminate mRNA with premature stop codons in it (Brogna and Wen, 2009).

Demo Box #3

Write something.

Demo Box #1

Our project has an advantage from a safety perspective in that it is largely cell-free. The process of BabbleBrick assembly only involves the magnetic beads, enzymes and, of course, the Bricks themselves. Our constructs can then be inserted into plasmids and stored in test tubes, and will actually be more stable outside of cells rather than inside them due to spontaneous mutations and replication errors that occur within cells (Allentoft et al., 2012; Lee et al., 2012).

Demo Box #2

Even if one were to insert a BabbleBlock into a cell to amplify, we took precautions so that it will not affect the cell’s function. To that end, we inserted stop codons in all three reading frames of our BabbleBricks, and in both directions, so that if our artificially constructed DNA were to somehow be transcribed, this will not result in nonsensical peptide chains that might interfere with normal cellular processes. Nonsense polypeptides are not well-tolerated in cells and so there are innate cellular mechanisms that eliminate mRNA with premature stop codons in it (Brogna and Wen, 2009).

Demo Box #3

Write something.

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