Difference between revisions of "Team:Exeter/Parts"

Revision as of 16:04, 10 October 2016

Parts

Description KillerRed KillerOrange Lysozyme

Discription

KillerRed

Name

pT7- E. coli optimised - KillerRed (EOKR)

Description

KillerRed is a red fluorescent protein that generates reactive oxygen species after illumination with light between the wavelengths of 540-580nm[1].

We further characterised this kill switch by illuminating induced cultures 24 hours after induction with IPTG as well as uninduced cultures. After 6 hours in the light box CFU’s were counted to determine if the kill switch was successful. This was also reproduced on cultures grown in a ministat for 120 and 168 hours to test how long the kill switch remains functional. The full protocol can be found here(link to protocol) and the results can be found here (link results).

The mechanism by which KillerRed kills cells isn’t fully understood yet

Here we are submitting KillerRed as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).

The sequence for KillerRed protein coding region can be found here (BBa_K1141002)

Biobrick Code

BBa_K1914003

KillerOrange

LysozymeC