Difference between revisions of "Team:Exeter/Parts"

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                <p id="pp">The aim of our project was to extensively test the viability of different of kill switches across an array of variables.  We focused on the ones that have already been used by previous teams (Pekin 2014, Oxford 2015) as well as a KillerRed homologue KillerOrange. This will allow future teams to more accurately chose a kill switch that suits their project the best as we have providing better understanding on the durability of the different kill switches as well as their efficiency. </p>
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                <p id="pp">We submitted a total of X new parts all of which are BioBrick compatible and codon optimised for E. coli strain K12. See list below for more details. </p>
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KillerOrange
 
KillerOrange
 
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                <h6>Name:</h6>
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                <p id="pp">pT7- E. coli optimised - KillerOrange (EOKO)</p>
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                <h6>Description:</h>
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                <p id="pp">KillerOrange is a mutant of the fluorescent protein KillerRed (BBa_K1141002, BBa_K1491015) activated by blue and green light. It carries a tryptophan-based chromophore that is novel for photosensitizers [2]. </p>
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                <p id="pp">KillerOrange has an excitation maximum of 512 nm and emission maximum at 555 nm and its absorbance spectrum has two peaks, at 455 and 514 nm. <p>
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                <p id="pp">It is believed that was confers KillerOrange's ability to generate reactive oxygen species depends on a water-filler channel reaching the chromophore area from the end cap of the β-barrel [3].</p>
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                <p id="pp">The mechanism by which KillerOrange kills cells isn’t fully understood yet[3]. </p>
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                <p id="pp">We characterised this part in the same way as KillerRed (link to protocol), and the results of which can be found here (link to results) </p>
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                <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
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                <p id="pp">The sequence for KillerOrange protein coding region can be found here (BBa_K1914000)</p>
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                <h6> Biobrick Code </h6>
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                <p id="pp">BBa_K1914001</p>
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Revision as of 16:08, 10 October 2016