Difference between revisions of "Tracks/Measurement/Interlab study/Flow Cytometry Overview"

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<center>This page is under active development and is in draft form.</center>
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<h3><a href="http://parts.igem.org/Help:InterLab_Measurement_Kit">Go to InterLab Measurement Kit Information Page</a></h3>
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In order to carry out this measurement protocol, you will need:
 
In order to carry out this measurement protocol, you will need:
 
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<li>A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP.  This channel will typically have a 488nm laser an a 530/30 filter, but the details may vary.</li>
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<li>A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP.  This channel will typically have a 488nm laser and a 530/30 filter, but the details may vary.</li>
 
<li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li>
 
<li>Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for. </li>
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<li>Email your completed Excel file to <i>measurement (AT) igem (DOT) org</i> [note: the empty template file is provided below]</li>
 
<li>Email your completed Excel file to <i>measurement (AT) igem (DOT) org</i> [note: the empty template file is provided below]</li>
 
<li>Fill in the <b>Flow Cytometer Form</b> provided at the bottom of this page</li>
 
<li>Fill in the <b>Flow Cytometer Form</b> provided at the bottom of this page</li>
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<li>Create an InterLab study page on your team wiki (please use this format: <i>https://2016.igem.org/Team:YourTeamName/InterLab</i>)
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[[Excel File Template]]
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Download the Excel Template file here: <a href="https://static.igem.org/mediawiki/2016/9/99/TeamName_iGEM2016_Flow_Cytometry_Workbook.xls">TeamName_iGEM2016_Flow_Cytometry_Worksheet.xls</a>
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You can see a filled-in example of the worksheet <a href="https://static.igem.org/mediawiki/2016/7/76/Flow_cytometry_workbook_example.xls">here</a>
 
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<b>Important!</b> If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:
 
<b>Important!</b> If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:
 
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       Flow Cytometer Form
 
       Flow Cytometer Form
 
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Latest revision as of 16:45, 21 June 2016




Go to InterLab Measurement Kit Information Page

Measurement with Flow Cytometry

This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.

In order to carry out this measurement protocol, you will need:

  • A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser and a 530/30 filter, but the details may vary.
  • Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for.


All teams using a flow cytometer to measure GFP must finish the following items to fulfill the InterLab:
  1. Email your completed Excel file to measurement (AT) igem (DOT) org [note: the empty template file is provided below]
  2. Fill in the Flow Cytometer Form provided at the bottom of this page
  3. Create an InterLab study page on your team wiki (please use this format: https://2016.igem.org/Team:YourTeamName/InterLab)


Download the Excel Template file here: TeamName_iGEM2016_Flow_Cytometry_Worksheet.xls

You can see a filled-in example of the worksheet here

Important! If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:

Flow Cytometer Form