Difference between revisions of "Team:Exeter/Parts"
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<p id="pp">KillerRed is a red fluorescent protein that generates reactive oxygen species after illumination with light between the wavelengths of 540-580nm[1].</p> | <p id="pp">KillerRed is a red fluorescent protein that generates reactive oxygen species after illumination with light between the wavelengths of 540-580nm[1].</p> | ||
| − | <p id="pp">We further characterised this kill switch by illuminating induced cultures 24 hours after induction with IPTG as well as uninduced cultures. After 6 hours in the light box CFU’s were counted to determine if the kill switch was successful. This was also reproduced on cultures grown in a ministat for 120 and 168 hours to test how long the kill switch remains functional. The full protocol can be found <a href="# | + | <p id="pp">We further characterised this kill switch by illuminating induced cultures 24 hours after induction with IPTG as well as uninduced cultures. After 6 hours in the light box CFU’s were counted to determine if the kill switch was successful. This was also reproduced on cultures grown in a ministat for 120 and 168 hours to test how long the kill switch remains functional. The full protocol can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_5">here</a> and the results can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a>. </p> |
<p id="pp">The mechanism by which KillerRed kills cells isn’t fully understood yet</p> | <p id="pp">The mechanism by which KillerRed kills cells isn’t fully understood yet</p> | ||
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<p id="pp">The mechanism by which KillerOrange kills cells isn’t fully understood yet[3]. </p> | <p id="pp">The mechanism by which KillerOrange kills cells isn’t fully understood yet[3]. </p> | ||
| − | <p id="pp">We characterised this part in the same way as KillerRed ( | + | <p id="pp">We characterised this part in the same way as KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_5">protocol</a>), and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p> |
<p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | ||
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<p id="pp"> We are submitting this Lysozyme C as a composite part under a T7 promoter, an Elowitz ribosome binding site (biobrick code) and a double terminator (BBa_B0015). We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm. The sequence for just Lysozyme C can be found here (biobrick code).</p> | <p id="pp"> We are submitting this Lysozyme C as a composite part under a T7 promoter, an Elowitz ribosome binding site (biobrick code) and a double terminator (BBa_B0015). We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm. The sequence for just Lysozyme C can be found here (biobrick code).</p> | ||
| − | <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed ( | + | <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_5">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p> |
<p id="pp">Here we are submitting lysozymeC as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | <p id="pp">Here we are submitting lysozymeC as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | ||
Revision as of 16:22, 10 October 2016
