Difference between revisions of "Team:SCAU-China/Model"

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{{SCAU-China}}
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<h3>★ ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#SpecialPrizes">Best Model award</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/b/b2/T--SCAU-China--logo_2.png"></a>
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  <li><a href="https://2016.igem.org/Team:SCAU-China">HOME</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Team">TEAM</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Project">PROJECT</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/PART">PART</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Result">RESULT</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/NOTEBOOK">NOTEBOOK</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/HP">HUMAN PRACTICE</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Safety">SAFETY</a></li>
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      <li><a href="#vecter" class="on">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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<h2> Modeling</h2>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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<h5> Inspiration </h5>
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      </ul>
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<p>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/3/31/T--SCAU-China--img_1.jpg"></div>
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Here are a few examples from previous teams:
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      <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
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</p>
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Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
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<ul>
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<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a></li>
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<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">Waterloo 2014</a></li>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="tissue"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue" class="on">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <img src="https://static.igem.org/mediawiki/2016/6/61/T--SCAU-China--img_2.jpg"><br>
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      <img src="https://static.igem.org/mediawiki/2016/4/4c/T--SCAU-China--img_3.jpg"><br>
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      <img src="https://static.igem.org/mediawiki/2016/7/75/T--SCAU-China--img_4.jpg">
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      <a id="pcr"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr" class="on">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <img src="https://static.igem.org/mediawiki/2016/0/0f/T--SCAU-China--img_5.jpg"><br>
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      <img src="https://static.igem.org/mediawiki/2016/b/b3/T--SCAU-China--img_6.jpg">
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      </div>     
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    <div class="vecter">
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      <ul class="nav">
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      <a id="phenotype"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype" class="on">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <img src="https://static.igem.org/mediawiki/2016/9/94/T--SCAU-China--image014.jpg">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/f/f6/T--SCAU-China--image016.png">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/0/02/T--SCAU-China--image015.png"><br><br>
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      <img src="https://static.igem.org/mediawiki/2016/8/8a/T--SCAU-China--image020.jpg">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/8/88/T--SCAU-China--image022.png">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/9/93/T--SCAU-China--image018.png">
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      </div>     
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    <div class="vecter">
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      <ul class="nav">
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      <a id="hplc"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc" class="on">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/d/dc/T--SCAU-China--image024.png"></div>
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      <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
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HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
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Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div> 
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/0/0b/T--SCAU-China--image026.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/4/42/T--SCAU-China--image028.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/8/84/T--SCAU-China--image030.png"></div>   
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="modeling"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling" class="on">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/2/2e/T--SCAU-China--image032.png"></div>
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      <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div> 
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</body>
 
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Revision as of 09:06, 11 October 2016

Fig.1 Not I digestion of constructs cotaining different numbers of genes. Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.



     

     
Fig.n HPLC analysis diagram of standard samples of astaxanthin(a); HPLC analysis diagram of samples extract from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);

Compared with the standard samples with HPLC analysis diagram, you can see the sample extract from rice and standard samples have the same peak at the same time, show that the transgenic rice seed which carry the four genes expressed astaxanthin. Results indicate that we are successful synthesize astaxanthin by rice endosperm synthetic .
Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .