Difference between revisions of "Team:SCAU-China/Demonstrate"

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{{SCAU-China}}
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<style type="text/css">
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#sideMenu, #top_title {display:none;}
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<p>Here you can describe the results of your project and your future plans. </p>
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#content { padding:0px; width:100%; margin-top:0px; margin-left:0px;}
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<h5>What should this page contain?</h5>
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<link href="https://2016.igem.org/Team:SCAU-China/CSS/result?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
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<ul>
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</head>
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<li> Clearly and objectively describe the results of your work.</li>
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<body>
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<li> Future plans for the project </li>
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<div id="page">
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<li> Considerations for replicating the experiments </li>
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  <div id="hd">
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</ul>
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  <div class="header index_bg">
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<div class="menu">
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  <ul>
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  <div class="logo">
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/b/b2/T--SCAU-China--logo_2.png"></a>
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  <a href="#"><img src="https://static.igem.org/mediawiki/2016/e/e3/T--SCAU-China--logo_3.png"></a></div>
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<li><a href="https://2016.igem.org/Team:SCAU-China">HOME</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Team">TEAM</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Project">PROJECT</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/PART">PART</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Result">RESULT</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/NOTEBOOK">NOTEBOOK</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/HP">HUMAN PRACTICE</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Safety">SAFETY</a></li>
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</ul>
 
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  <div class="triangle"><a class="click"></a></div>
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    <div class="vecter">
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<h5> Project Achievements </h5>
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      <ul class="nav">
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      <a id="vecter"></a>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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      <li><a href="#vecter" class="on">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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<ul>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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<li>A list of linked bullet points of the successful results during your project</li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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      <li><a href="#hplc">HPLC</a></li>
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</ul>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/3/31/T--SCAU-China--img_1.jpg"></div>
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      <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
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Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
 
</div>
 
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<div class="column half_size" >
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    <div class="vecter">
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      <ul class="nav">
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<h5>Inspiration</h5>
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      <a id="tissue"></a>
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<p>See how other teams presented their results.</p>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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<ul>
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      <li><a href="#tissue" class="on">Tissue Culture</a></li>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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      <li><a href="#hplc">HPLC</a></li>
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</ul>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <img src="https://static.igem.org/mediawiki/2016/6/61/T--SCAU-China--img_2.jpg"><br>
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      <img src="https://static.igem.org/mediawiki/2016/4/4c/T--SCAU-China--img_3.jpg"><br>
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      <img src="https://static.igem.org/mediawiki/2016/7/75/T--SCAU-China--img_4.jpg">
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="pcr"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
 +
      <li><a href="#pcr" class="on">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <img src="https://static.igem.org/mediawiki/2016/0/0f/T--SCAU-China--img_5.jpg"><br>
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      <img src="https://static.igem.org/mediawiki/2016/b/b3/T--SCAU-China--img_6.jpg">
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="phenotype"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype" class="on">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img">
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      <img src="https://static.igem.org/mediawiki/2016/9/94/T--SCAU-China--image014.jpg">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/f/f6/T--SCAU-China--image016.png">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/0/02/T--SCAU-China--image015.png"><br><br>
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      <img src="https://static.igem.org/mediawiki/2016/8/8a/T--SCAU-China--image020.jpg">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/8/88/T--SCAU-China--image022.png">&nbsp;&nbsp;
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  <img src="https://static.igem.org/mediawiki/2016/9/93/T--SCAU-China--image018.png">
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      </div>     
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="hplc"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
 +
      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
 +
      <li><a href="#phenotype">Phenotype of seeds</a></li>
 +
      <li><a href="#hplc" class="on">HPLC</a></li>
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      <li><a href="#modeling">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/d/dc/T--SCAU-China--image024.png"></div>
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      <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
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HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
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Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div> 
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/0/0b/T--SCAU-China--image026.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/4/42/T--SCAU-China--image028.png"></div>
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<div class="img"><img src="https://static.igem.org/mediawiki/2016/8/84/T--SCAU-China--image030.png"></div>   
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    </div>
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    <div class="vecter">
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      <ul class="nav">
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      <a id="modeling"></a>
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      <li><a href="#vecter">Vecter Constraction</a></li>
 +
      <li><a href="#tissue">Tissue Culture</a></li>
 +
      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
 +
      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <li><a href="#modeling" class="on">Modeling</a></li>
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      </ul>
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/2/2e/T--SCAU-China--image032.png"></div>
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      <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div> 
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    </div>
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  </div>
 
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</body>
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Revision as of 09:14, 11 October 2016

Fig.1 Not I digestion of constructs cotaining different numbers of genes. Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.



     

     
Fig.n HPLC analysis diagram of standard samples of astaxanthin(a); HPLC analysis diagram of samples extract from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of samples extract from HG-WT rice (c);

Compared with the standard samples with HPLC analysis diagram, you can see the sample extract from rice and standard samples have the same peak at the same time, show that the transgenic rice seed which carry the four genes expressed astaxanthin. Results indicate that we are successful synthesize astaxanthin by rice endosperm synthetic .
Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .