Difference between revisions of "Team:Exeter/Integrated Practices"

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                 <p id="pp">By interviewing academic and industry researchers about their understanding of kill switches, we could analyse whether they are effective biosafety mechanisms and if they are appropriate for use in either industry or academic research.</p>
 
                 <p id="pp">By interviewing academic and industry researchers about their understanding of kill switches, we could analyse whether they are effective biosafety mechanisms and if they are appropriate for use in either industry or academic research.</p>
 
                  
 
                  
                 <p id="pp">Dr Tom Ellis influenced the design of our kill switches by corroborating our theory that multiple kill switches in one system would reduce the error rate significantly - creating a fail safe, in the same way that broad spectrum antibiotics significantly reduces the chances of failure. We had planned on making an operon of KillerOrange and KillerRed, to both broaden the spectrum of light at which the reactive oxygen species are produced, but also significantly reduce the error rates of one. Dr Ellis argued that multiple kill switches in one system might have “less than one in a billion escape rates”.</p>
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                 <p id="pp">Dr Tom Ellis influenced the design of our kill switches by corroborating our theory that multiple kill switches in one system would reduce the error rate significantly - creating a fail safe, in the same way that broad spectrum antibiotics significantly reduces the chances of failure. We had planned on making an operon of KillerOrange and KillerRed, to both broaden the spectrum of light at which the reactive oxygen species are produced, but also significantly reduce the error rates of one. Dr Ellis argued that multiple kill switches in one system might have <q style="padding-left:0px;padding-right:0px;">less than one in a billion escape rates</q>.</p>
 
                  
 
                  
 
                 <p id="pp">Due to time restraints with our project we decided to focus on providing significant analysis on the kill switches individually, however advice from Markus Geshater of Synthase Limited indicating that multiple kill switches in one system would be unfeasible and the different systems would need to be truly orthogonal to be effective. He explained that if in an industrial setting your synthetic system is producing a protein, then having one or multiple kill switches that are reliant on protein production would reduce the efficiency of production of your desired protein. Furthermore these kill switches could potentially be circumvented by the overexpression of a useful enzyme that is being commercially produced. Consequently, we felt assured in dropping the idea of a multiple kill switch system, because, as our kill switches rely heavily on protein production, and we want to provide analysis for future teams wanting to use kill switches in the future, if their synthetic systems relied on protein production, then our kill switches would be unfeasible. Instead we looked to design three distinct kill switches to test the efficiency of enzymatic, metabolic and DNA degradating mechanisms</p>
 
                 <p id="pp">Due to time restraints with our project we decided to focus on providing significant analysis on the kill switches individually, however advice from Markus Geshater of Synthase Limited indicating that multiple kill switches in one system would be unfeasible and the different systems would need to be truly orthogonal to be effective. He explained that if in an industrial setting your synthetic system is producing a protein, then having one or multiple kill switches that are reliant on protein production would reduce the efficiency of production of your desired protein. Furthermore these kill switches could potentially be circumvented by the overexpression of a useful enzyme that is being commercially produced. Consequently, we felt assured in dropping the idea of a multiple kill switch system, because, as our kill switches rely heavily on protein production, and we want to provide analysis for future teams wanting to use kill switches in the future, if their synthetic systems relied on protein production, then our kill switches would be unfeasible. Instead we looked to design three distinct kill switches to test the efficiency of enzymatic, metabolic and DNA degradating mechanisms</p>

Revision as of 10:30, 11 October 2016