Difference between revisions of "Team:Aix-Marseille/Composite Part"

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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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    <!-- end hearo section -->
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<div class="container-fluid">
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<div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">Parts</div>
 +
 
 +
      <!-- start section 1 -->
 +
    <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
 +
   
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-6 left">
 +
    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
 +
</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:
 +
<ul><br/>
 +
<li>-T7 promoter with RBS (Bba_K525998) and optimized Laccase from <i>T.Thermophilus</i> (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)</li><br/>
 +
<li>-T7 promoter with RBS and optimized Laccase from <i>T.Thermophilus</i>, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)</li>
 +
</ul>
 +
</div></p></span>
 +
 
 +
 
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
                          <img src="https://static.igem.org/mediawiki/2015/3/35/Our.png" class="img-responsive" width="475" height="325">
 +
                         
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 +
   
 +
    </div>
 +
    </div>
 +
            <div class="space30"></div>
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    </div>
 +
 
 +
    </section>
 +
    <div class="clearfix"></div>
 +
 
 +
      <!-- start section 2 -->
 +
  <section style="padding:30px 0px 50px;" class="arrow_box" id="team">
 +
   
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-6 left">
 +
    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks we designed</div>
 +
</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:<br/>
 +
<ul>
 +
<li>-Cytochrome C <i>Synechocystis sp</i> (BBa_K1601001), a cyanobacteria that suggests a cytochrome c </li><br/>
 +
<li>-Cytochrome C of <i>S.oneidensis</i> (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.</li><br/>
 +
<li>-Heme A from <i>E.coli</i> K12 (BBa_K1601002)that enables the heme overproduction</li><br/>
 +
<li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the <i>S.oneidensis</i> CytC translocation into the periplasm using TAT system (BBa_S05319)</li><br/>
 +
<li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the <i>Synechocystis sp</i> CytC translocation into the periplasm using TAT system (BBa_S05320)</li><br/>
 +
</ul>
 +
</div></p></span>
 +
 
 +
 
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
 +
<br/><br/><br/><br/><br/><br/>
 +
                          <img src="https://static.igem.org/mediawiki/2015/c/cd/Idqfgfg.png" class="img-responsive" width="1000" height="400">
 +
                         
 +
 
 +
   
 +
    </div>
 +
    </div>
 +
            <div class="space30"></div>
 +
    </div>
 +
 
 +
    </section>
 +
    <!-- start section 3 -->
 +
<section style="padding:30px 0px 50px;" class="arrow_box" id="team">
 +
   
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-6 left">
 +
    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle"> Linkers we designed</div>
 +
</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="font-family:Courier New">We designed some linker to facilitate the meeting of the laccase and the cytochrom C
 +
</p></span></div>
 +
<p class="space20"><div align="justify"><span style ="font-family:Courier New">
 +
<ul>
 +
<li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>S.oneidensis (BBa_K1601008)</i></li> <br/>
 +
<li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>T.Thermophilus</i> and CytC <i>P.denitrificans (BBa_K1601011)</i></li><br/>
 +
<li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) <i>T.Thermophilus</i> and CytC <i>Synechocystis sp(BBa_K1601013)</i></li><br/>
 +
<li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>P.denitrificans (BBa_K1601009)</i></li><br/>
 +
<li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>Synechocystis sp (BBa_K1601010)</i></li><br/>
 +
<li style="list-style-type:circle;">Rigid and structured linker designed to link (or connect) Laccase <i>E.coli</i> and CytC <i>S.oneidensis(BBa_K1601012)</i></li>
 +
</ul> </div></p></span>
 +
 
 +
 
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
 +
<br/><br/><br/><br/><br/><br/>
 +
                            <img src="https://static.igem.org/mediawiki/2015/7/78/Idqfj%2Cg.png" class="img-responsive" width="600" height="1200">
 +
                         
 +
 
 +
   
 +
    </div>
 +
    </div>
 +
</div>
 +
            <div class="space30"></div>
 +
    </div>
 +
 
 +
    </section>
 +
<section style="padding:30px 0px 50px;" class="arrow_box" id="team">
 +
   
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-6 left">
 +
    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
 +
</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:<br/>
 +
<ul>
 +
    <li>-Yeast signal CXXCH from <i>Synechocystis sp</i> responsible for the S. oneidensis CytC translocation into the periplasm using TAT system</li>
 +
    <li>-T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)</li>
 +
    <li>-T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)</li>
 +
    <li>-Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)</li>
 +
    <li>-Yeast signal CXXCH (BBa_K1601006)</li>
 +
    <li>-Cytochrome C P.Denitrificans (BBa_K1601007)</li>
 +
    <li>-Cytochrome C Synechocystis sp - His Tag (BBa_S05314)</li>
 +
    <li>-Cytochrome C S.oneidensis - HisTag (BBa_S05315)</li>
 +
</ul>
 +
</div></p></span>
 +
 
 +
 
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
 +
<br/><br/><br/><br/><br/>
 +
                          <img src="https://static.igem.org/mediawiki/2015/6/60/Qsffff.png" class="img-responsive" width="475" height="325">
 +
                         
 +
 
 +
   
 +
    </div>
 +
    </div>
 +
            <div class="space30"></div>
 +
    </div>
 +
 
 +
    </section>
 +
<section style="padding:30px 0px 50px;" class="arrow_box" id="team">
 +
   
 +
    <div class="container">
 +
    <div class="row">
 +
    <div class="col-md-6 left">
 +
    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
 +
</h2></span></span>
 +
    <div class="space30"></div>
 +
    <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:<br/>
 +
<ul>
 +
    <li>Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)</li>
 +
    <li>Cytochrome C P.Denitrificans - His Tag (BBa_S05317)</li>
 +
    <li>Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)</li>
 +
    <li>T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)</li>
 +
</ul>
 +
</div></p></span>
 +
    </div>
 +
    <div class="col-md-5 col-md-offset-1  col-sm-offset-1  space30">
 +
<br/><br/><br/><br/><br/>
 +
                          <img src="https://static.igem.org/mediawiki/2015/a/a7/Qsffqfqdf.png" class="img-responsive" width="475" height="325">
 +
                         
 +
 
 +
   
 +
    </div>
 +
    </div>
 +
            <div class="space30"></div>
 +
    </div>
 +
 
 +
    </section>
 +
    <div class="clearfix"></div>   
 +
 
 +
    </section>   
 +
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                </div>
 +
    </div>
 +
    </div>
 +
 
 +
    </section>
 +
      <div class="clearfix"></div>
 +
      <!-- start sponsors section -->
 +
<section style="padding:50px 0px;" class="arrow_box-2">
 +
       
 +
        <div class="container">
 +
            <div class="row">
 +
                <div class="col-md-12 text-center">
 +
                    <h2 class="gray">SPONSORS</h2>
 +
<ul class="list-inline space30 icon">
 +
  <li><a href="https://www.corning.com/worldwide/en/products/life-sciences.html">
 +
    <img src="https://static.igem.org/mediawiki/2015/8/87/Corninglogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.crous-aix-marseille.fr/social/fsdie-social">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/18/KlP5G8h.jpg"
 +
    class="img-grey mautomargin" width="200" align="center"></a></li>
 +
  <li><a href="http://www.cnrs.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/8/8e/CNRSlogo.jpg"
 +
    width="200" class="img-grey mautomargin" align="center"></a></li>
 +
  <li><a href="https://www.qiagen.com/fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/f/f1/Qialogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/38/EcfFf9Kw.png"
 +
    class="img-grey mautomargin" width="200" align="center" ></a></li>
 +
  <li><a href="http://polytech.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/10/ILFZ36R.png"
 +
    class="img-grey mautomargin" width="200" align="center"></a></li>
 +
  <li><a href="http://www.grandluminy.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/6/66/GandLlogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="https://www.eppendorf.com/FR-fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/db/Eppendorflogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.dutscher.com/frontoffice/home">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/10/Deutcherflogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www.mn-net.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/1b/MNlogo.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="https://fr.groupeonet.com/Onet-Proprete-et-Services">
 +
    <img src="https://static.igem.org/mediawiki/2015/3/3d/5tokNzS.jpg" width="200" align="center"
 +
    class="img-grey mautomargin"></a></li>
 +
  <li><a href="http://www3.gehealthcare.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/b/bd/GEHlogo.jpg" width="200"
 +
    class="img-grey mautomargin" align="center" ></a></li>
 +
  <li><a href="https://www.sigmaaldrich.com/france.html">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/41/PUNXaNj.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="http://www.starlab.de/int/?l=5">
 +
    <img src="https://static.igem.org/mediawiki/2015/1/13/StarLablogo.jpg" align="center"
 +
    class="img-grey mautomargin" width="200" height="100"></a></li>
 +
  <li><a href="http://fr.ambafrance-us.org/">
 +
    <img src="https://static.igem.org/mediawiki/2015/2/2b/M3ieC2R.jpg" align="center"
 +
    class="img-grey mautomargin" width="200" height="100"></a></li>
 +
  <li><a href="https://www.neb.com/">
 +
    <img src="https://static.igem.org/mediawiki/2015/a/a9/Ll3uagr.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="http://sciences.univ-amu.fr/">
 +
    <img src="https://static.igem.org/mediawiki/2015/4/4a/FtFgEFj.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
  <li><a href="https://eu.idtdna.com/site">
 +
    <img src="https://static.igem.org/mediawiki/2015/d/d9/IDTlogo.jpg" align="center"
 +
    class="img-grey mautomargin" width="200"></a></li>
 +
</ul>
 +
 
 +
                 
 +
                </div>
 +
 
 +
            </div>
 +
              <div class="space20"></div>
 +
        </div>
 +
    </section>
 +
      <!-- start footer -->
 +
    <footer>
 +
        <div class="container">
 +
            <div class="row">
 +
                <div class="col-md-4">
 +
                    <h3>Useful Links</h3>
 +
                    <ul class="f-menu space40">
 +
                          <li><a href="/Team:Aix-Marseille">Home</a></li>
 +
                        <li><a href="https://2015.igem.org/Main_Page">iGEM 2015</a></li>
 +
                    </ul>
 +
                </div>
 +
                <div class="col-md-4">
 +
                    <h3>Contact Us</h3>
 +
                    <ul class="f-menu space40">
 +
                     
 +
                        <br/>
 +
                        <li>Aix Marseille Université</li>
 +
                        <li>Marseille</li>
 +
                        <li>France</li>
 +
                    </ul>
 +
                </div>
 +
                <div class="col-md-4">
 +
                    <div class="f-text">
 +
                        <p class="fsize18 space10">Chew figth project, for the iGEM competition. See you soon in Boston !</p>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
 
 +
        </div>
 +
 
 +
 
 +
    </footer>
 +
 
 +
    <script type="text/javascript">
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          if (mq.matches) {
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            $('ul.navbar-nav li').addClass('hovernav');
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</body>
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</html>
  
−
<h4>Note</h4>
 
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
 
  
 
</div>
 
</div>

Revision as of 19:49, 11 October 2016