Difference between revisions of "Team:Aix-Marseille/Composite Part"
| Line 19: | Line 19: | ||
<li>RBS: Ribosome Binding Site</li> | <li>RBS: Ribosome Binding Site</li> | ||
<li>pARA/araC inductible promoter</li> | <li>pARA/araC inductible promoter</li> | ||
| − | <li>Lysine | + | <li>Lysine decarboxylase</li> |
</ul> | </ul> | ||
</p> | </p> | ||
| + | |||
| + | <h4><u>Part Composition:</u></h4> | ||
| + | |||
| + | <p> Lysine descarboxylase from <i>Streptomyces coelicolor</i> is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. | ||
| + | In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. | ||
| + | We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for <i>E.coli</i> and ordered the synthesis by addition of an inductible promoter.</p> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
