Difference between revisions of "Team:Aix-Marseille/Composite Part"

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In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.  
 
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.  
 
We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for <i>E.coli</i> and ordered the synthesis by addition of an inductible promoter.</p>
 
We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for <i>E.coli</i> and ordered the synthesis by addition of an inductible promoter.</p>
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<br><br>
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<h3 class="titrepage">BBa_K1622001</h3>
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<h4><u>Part Composition:</u><h4>
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<p>This part is a composite part composed of 2 Biobricks :
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<ul>
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  <li>BBa_K808030: NB-Esterase</li>
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  <li>BBa_K808007: TPA Transporter</li>
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</ul>
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</p>
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<br>
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<h4><u>NB-Esterase:</u></h4>
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<p>This enzyme catalyses the degradation of PET into Terephthalic acid (TPA) and Ethylene Glycol. It is the first and slowest reaction of our degradation chain. Indeed, it takes two weeks to degrade PET.</p>
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<p> We performed an enzymatic assay of NB-Esterase using 4-nitrophenylbutyrate as a substrate because it has a close chemical structure to PET and we figured that this enzyme digested pNP in our bacteria</p>
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<h4><u>TPA Transporter:</u></h4>
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<p>The NB-Esterase is present in the extracellular medium and cuts PET in this location. In order to be fully degraded, the TPA needs to be integrated to our chassis organism which is why we incorporated a transporter to our degradation operon.</p>
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<br>
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<h4><u>Part Assembly:</u></h4>
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<p> The subparts were assembled using standard BioBrick Assembly.</p>
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<br><br>
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</div>
 
</div>

Revision as of 20:38, 11 October 2016