Difference between revisions of "Team:Aix-Marseille/Composite Part"

Revision as of 21:55, 11 October 2016

Composite part

Composite parts we have designed

BBa_K1951004 : DesA producer

Part Composition:

This part is composed of 3 subparts :

Bba_I0500 : Ribosome Binding Site
Bba_B0034 : pARA/araC inductible promoter
Bba_K1951000 : Lysine decarboxylase

[[File:Aix Marseille--Prom FliC.jpeg]]

Lysine decarboxylase DesA:

Lysine descarboxylase from Streptomyces coelicolor is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for E.coli and ordered the synthesis by addition of an inductible promoter.

BBa_K1941008 : FliC E. coli producer

Part Composition:

This part is a composite part composed of 2 Biobricks :

BBa_K1951005: flagelln C

BBa_K880005: Strong promoter, strong RBS combination

Flagellin C from Escherichia coli

Flagellin C (FliC) protein from Escherichia coli strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...

We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110

Part Assembly:

The subparts were assembled using standard BioBrick Assembly.