Difference between revisions of "Team:Northwestern/06 30"

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     <h1>Thursday, June 30<sup>th</sup></h3>
 
     <h1>Thursday, June 30<sup>th</sup></h3>
 
     <h2>Agenda:</h2>
 
     <h2>Agenda:</h2>
 +
<ul>
 +
      <li>Constructs</li>
 +
      <li>Discuss things with Purdue</li>
 +
      <li>Protocol Practice- miniprep and digestion</li>
 +
      <li>Crowdfunding</li>
 +
      <li>Presentation practice</li>
 +
    </ul>
 +
    <h2>Results:</h2>
 +
    <ul>
 +
      <li>Nothing in the gel except the ladder was visible under UV light </li>
 +
      <li>The ImageJ program was not working</li>
 +
      <li>Left the gel out too long in the light and our SybrGreen degraded</li>
 +
    </ul>
 +
    <h2>Tasks:</h2>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>CSS studying</li>
 +
          <li>Tat &amp; Sec sequence selection</li>
 +
          <li>Miniprepped practice plasmids </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Wrote about things to discuss with Purdue </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Did the digest and ran the gel</li>
 +
          <li>Emailed Bernd about split Cas9 </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>ClyA construct</li>
 +
          <li>Miniprep practice </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Communicated w/sponsors (Promega and Invitrogen)</li>
 +
          <li>Worked on split Cas9 project, designed overlaps, need to check with Bradley </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
</article>
  
 
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       </div>
 
     </footer>
 
     </footer>
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<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
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         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>
 
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Revision as of 15:14, 12 October 2016

Notebook

Thursday, June 30th

Agenda:

  • Constructs
  • Discuss things with Purdue
  • Protocol Practice- miniprep and digestion
  • Crowdfunding
  • Presentation practice

Results:

  • Nothing in the gel except the ladder was visible under UV light
  • The ImageJ program was not working
  • Left the gel out too long in the light and our SybrGreen degraded

Tasks:

Michelle

  • CSS studying
  • Tat & Sec sequence selection
  • Miniprepped practice plasmids

Sam

  • Wrote about things to discuss with Purdue

Sara

  • Did the digest and ran the gel
  • Emailed Bernd about split Cas9

Tasfia

  • ClyA construct
  • Miniprep practice

Tyler

  • Communicated w/sponsors (Promega and Invitrogen)
  • Worked on split Cas9 project, designed overlaps, need to check with Bradley