Difference between revisions of "Team:Northwestern/07 12"

(Created page with "<html> <style> </style> <head> <meta charset="UTF-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-width, initial-...")
 
Line 21: Line 21:
 
     <h1>Tuesday, July 12<sup>th</sup></h3>
 
     <h1>Tuesday, July 12<sup>th</sup></h3>
 
     <h2>Agenda:</h2>
 
     <h2>Agenda:</h2>
 +
 +
    <ul>
 +
<li>Transform pSB1C3 &amp; miniprep</li>
 +
<li>PCR cleanup/Gel</li>
 +
    </ul>
 +
<h2>Tasks:</h2>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Jordan</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Talked about ethics with CTD kids</li>
 +
      <li>PCR cleanup: used nuclease-free water instead of elution buffer, final conc. ~55 ng/uL</li>
 +
      <li>Added lab notes to Experiment </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li> Made 1L of 1X TAE buffer:</li>
 +
          <ul><li>100 mL 10X TAE</li>
 +
          <li>Filled to 1L dH<sub>2</sub>O</li></ul>
 +
          <li>Made 100mL of 1% agarose:</li>
 +
          <ul><li>1 g agarose</li>
 +
          <li>100 mL of 1X TAE</li></ul>
 +
          <li>Gel prep</li>
 +
          <ul>
 +
            <li>3 uL SybrGreen</li>
 +
            <li>Allowed the gel to cool for a long time (~1.5 hours)</li>
 +
          <li>Used 5 uL of PCR product from linearizing the backbone and 1 uL purple loading dye</li>
 +
          <li>The agarose was still unusually soft</li></ul>
 +
          <li>Website research</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Paul</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Outreach: Genedrive presentation</li>
 +
      <li>Transformed pSB1C3+Tet</li>
 +
      <li>PCR cleanup of linearized Tet backbone for Cas9 insertions </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Did the outreach + made the outreach doc</li>
 +
          <li>Researched <em>S. aureus</em> gRNA</li>
 +
          <li>Responded to Rose</li>
 +
          <li>Refined CSS talents</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Sara </p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Outreach at Roycemore</li>
 +
      <li>Emailed Bernd asking for empty backbones for saCas9 and spCas9</li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="row">
 +
  <div class="col-sm-2">
 +
    <p>Shu</p>
 +
  </div>
 +
  <div class="col-sm-10">
 +
    <ul>
 +
      <li>Updated our logo + graphics design research </li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Wrote/started lab notes on Experiment</li>
 +
          <li>Read up on mechanism of CRISPR/Cas9</li>
 +
          <li>Started a spreadsheet for iGEM parts we’re using</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Gel prep</li>
 +
          <li>Read up on saCas9 on protein structure/sgRNA design</li>
 +
          <li>Began designing gRNA gblock</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <h2>Results:</h2>
 +
    <p>Paul’s Tet-plasmid linearization worked, the others did not</p>
 +
  </article>
  
 
<footer id="nav">
 
<footer id="nav">
Line 29: Line 142:
 
       </div>
 
       </div>
 
     </footer>
 
     </footer>
−
  </article>
+
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
<script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/jquery?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>
 
         <script type="text/javascript" src="https://2016.igem.org/Team:Northwestern/libraries/bootstrap?action=raw&ctype=text/javascript"></script>

Revision as of 16:27, 12 October 2016

Notebook

Tuesday, July 12th

Agenda:

  • Transform pSB1C3 & miniprep
  • PCR cleanup/Gel

Tasks:

Jordan

  • Talked about ethics with CTD kids
  • PCR cleanup: used nuclease-free water instead of elution buffer, final conc. ~55 ng/uL
  • Added lab notes to Experiment

Michelle

  • Made 1L of 1X TAE buffer:
    • 100 mL 10X TAE
    • Filled to 1L dH2O
  • Made 100mL of 1% agarose:
    • 1 g agarose
    • 100 mL of 1X TAE
  • Gel prep
    • 3 uL SybrGreen
    • Allowed the gel to cool for a long time (~1.5 hours)
    • Used 5 uL of PCR product from linearizing the backbone and 1 uL purple loading dye
    • The agarose was still unusually soft
  • Website research

Paul

  • Outreach: Genedrive presentation
  • Transformed pSB1C3+Tet
  • PCR cleanup of linearized Tet backbone for Cas9 insertions

Sam

  • Did the outreach + made the outreach doc
  • Researched S. aureus gRNA
  • Responded to Rose
  • Refined CSS talents

Sara

  • Outreach at Roycemore
  • Emailed Bernd asking for empty backbones for saCas9 and spCas9

Shu

  • Updated our logo + graphics design research

Tasfia

  • Wrote/started lab notes on Experiment
  • Read up on mechanism of CRISPR/Cas9
  • Started a spreadsheet for iGEM parts we’re using

Tyler

  • Gel prep
  • Read up on saCas9 on protein structure/sgRNA design
  • Began designing gRNA gblock

Results:

Paul’s Tet-plasmid linearization worked, the others did not