Difference between revisions of "Team:XJTLU-CHINA/Parts"
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| − | {{XJTLU-CHINA}} | + | {{:Team:XJTLU-CHINA/header}} |
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<html> | <html> | ||
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| + | <link rel="stylesheet" href="https://2016.igem.org/Team:XJTLU-CHINA/css/template/reset?action=raw&ctype=text/css"> | ||
<head> | <head> | ||
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| + | table { | ||
| + | max-width: 500px; | ||
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| + | color: #002bb8; | ||
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</head> | </head> | ||
| − | + | <body> | |
| + | <div align="center" style="vertical-align:bottom"> | ||
<table width=90% class="parts text-center"> | <table width=90% class="parts text-center"> | ||
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<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
| − | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906000"> BBa-K1906000 </a></td> |
</tr> | </tr> | ||
| Line 45: | Line 58: | ||
<tr> | <tr> | ||
<td align="CENTER">Tu</td> | <td align="CENTER">Tu</td> | ||
| − | <td align="CENTER"> <a href="http://parts.igem.org/Part: | + | <td align="CENTER"> <a href="http://parts.igem.org/Part:BBa_K1906001"> BBa-K1906001 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">Ts</td> | <td align="CENTER">Ts</td> | ||
| − | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="hhttp://parts.igem.org/Part:BBa_K1906002"> BBa-K1906002 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">replicase</td> | <td align="CENTER">replicase</td> | ||
| − | <td align="CENTER"><a href="http://parts.igem.org/Part: | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906003">BBa-K1906003 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">intron a</td> | <td align="CENTER">intron a</td> | ||
| − | <td align="CENTER"><a href="http://parts.igem.org/Part: | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906004">BBa-K1906004 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">intron b</td> | <td align="CENTER">intron b</td> | ||
| − | <td align="CENTER"><a href="http://parts.igem.org/Part: | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906005">BBa-K1906005 </a></td> |
</tr> | </tr> | ||
| Line 75: | Line 88: | ||
<tr> | <tr> | ||
<td align="CENTER">akb</td> | <td align="CENTER">akb</td> | ||
| − | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906007">BBa-K1906007 </a></td> |
</tr> | </tr> | ||
| Line 85: | Line 98: | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
| − | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906001"> BBa-K1906001 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
| − | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906002"> BBa-K1906002 </a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td align="CENTER">maturase</td> | <td align="CENTER">maturase</td> | ||
| − | <td align="CENTER"><a href=" | + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906003"> BBa-K1906003 </a></td> |
</tr> | </tr> | ||
| + | <tr> | ||
| + | <td colspan="2" align="CENTER"> | ||
| + | <h4> <b>Function </b> </h4> | ||
| + | </td> | ||
| + | <tr> | ||
| + | <td colspan="2" > | ||
| + | <p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool </p> | ||
| + | </td> | ||
| + | <tr> | ||
| + | <td colspan="2" align="CENTER"> | ||
| + | <h4> <b>Part Collection 2 </b> </h4> | ||
| + | </td> | ||
| + | <tr> | ||
| + | <td align="CENTER">maturase</td> | ||
| + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906000"> BBa-K1906000 </a></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td align="CENTER">intron a</td> | ||
| + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906004">BBa-K1906004 </a></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td align="CENTER">intron b</td> | ||
| + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906005"> BBa-K1906005 </a></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td align="CENTER">akb</td> | ||
| + | <td align="CENTER"><a href="http://parts.igem.org/Part:BBa_K1906007">BBa-K1906007 </a></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td colspan="2" align="CENTER"> | ||
| + | <h4> <b>Function </b> </h4> | ||
| + | </td> | ||
| + | |||
| + | <tr> | ||
| + | <td colspan="2" > | ||
| + | <p> Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction </p> | ||
| + | </td> | ||
| + | </table> | ||
| + | </div> | ||
| + | |||
| + | </body> | ||
</html> | </html> | ||
| + | |||
| + | {{:Team:XJTLU-CHINA/footer}} | ||
Latest revision as of 16:32, 12 October 2016
PART NAME |
PART NUMBER |
Basic Part |
|
| maturase | BBa-K1906000 |
| Tu | BBa-K1906001 |
| Ts | BBa-K1906002 |
| replicase | BBa-K1906003 |
| intron a | BBa-K1906004 |
| intron b | BBa-K1906005 |
Composite Part |
|
| akb | BBa-K1906007 |
Part Collection 1 |
|
| maturase | BBa-K1906001 |
| maturase | BBa-K1906002 |
| maturase | BBa-K1906003 |
Function |
|
|
One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool |
|
Part Collection 2 |
|
| maturase | BBa-K1906000 |
| intron a | BBa-K1906004 |
| intron b | BBa-K1906005 |
| akb | BBa-K1906007 |
Function |
|
|
Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction |
|
