Difference between revisions of "Team:XJTLU-CHINA/Parts"
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<p> Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction </p> | <p> Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction </p> | ||
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Latest revision as of 16:32, 12 October 2016
PART NAME |
PART NUMBER |
Basic Part |
|
| maturase | BBa-K1906000 |
| Tu | BBa-K1906001 |
| Ts | BBa-K1906002 |
| replicase | BBa-K1906003 |
| intron a | BBa-K1906004 |
| intron b | BBa-K1906005 |
Composite Part |
|
| akb | BBa-K1906007 |
Part Collection 1 |
|
| maturase | BBa-K1906001 |
| maturase | BBa-K1906002 |
| maturase | BBa-K1906003 |
Function |
|
|
One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool |
|
Part Collection 2 |
|
| maturase | BBa-K1906000 |
| intron a | BBa-K1906004 |
| intron b | BBa-K1906005 |
| akb | BBa-K1906007 |
Function |
|
|
Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction |
|
