Difference between revisions of "Team:Aix-Marseille/Composite Part"
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====Part Composition:==== | ====Part Composition:==== | ||
This part is composed of 3 subparts : | This part is composed of 3 subparts : | ||
| + | * Bba_I0500 : Ribosome Binding Site | ||
| + | * Bba_B0034 : pARA/araC inductible promoter | ||
| + | * Bba_K1951000 : Lysine decarboxylase | ||
| + | [[File:Aix_Marseille--Prom_FliC.jpeg.jpg|200px|right]] | ||
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| + | ====Lysine decarboxylase DesA:==== | ||
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| + | Lysine descarboxylase from ''Streptomyces coelicolor'' is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. | ||
| + | In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. | ||
| + | We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for ''E.coli'' and ordered the synthesis by addition of an inductible promoter. | ||
{{:Team:Aix-Marseille/Template-Footer}} | {{:Team:Aix-Marseille/Template-Footer}} | ||
