===Pathway of the desferrioxamine B biosynthesis===

===Pathway of the desferrioxamine B biosynthesis===

[[File:T--Aix-Marseille--desABCD.jpeg|600px|left|thumb|Desferrioxamine B pathway]]  

[[File:T--Aix-Marseille--desABCD.jpeg|600px|left|thumb|Desferrioxamine B pathway]]  

Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. ''E. coli'' was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive, considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).   

Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. ''E. coli'' was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive, considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).   

Our strategy for the siderophore mobilisation results in two main steps :

Our strategy for the siderophore mobilisation results in two main steps :

* Transformation of E.Coli with an inductible plasmide holding the ''des'' cluster

* Transformation of E.Coli with an inductible plasmide holding the ''des'' cluster

* Induction and production of the desferrioxamine B

* Induction and production of the desferrioxamine B

Then, the produced siderophore would be purified and transfered in the recuperation solution containing the platinum and other metals. Therefore, those metals must be recruit by our purified siderophores. Though those siderophores would be import using a sowing of ''Streptomyces coelicolor''. Then simple centrifugation and combustion will concentrate the solution in metal a first time.

Then, the produced siderophore would be purified and transfered in the recuperation solution containing the platinum and other metals. Therefore, those metals must be recruit by our purified siderophores. Though those siderophores would be import using a sowing of ''Streptomyces coelicolor''. Then simple centrifugation and combustion will concentrate the solution in metal a first time.