Difference between revisions of "Team:Aix-Marseille/Results"

Revision as of 14:23, 13 October 2016

MObilisation result

===Proof of protein production===

Production of every enzymes involved in the pathway has been shown by a SDS page and Comassie Blue. We compared the production of proteins in different background :

- E. coli Tg1 wuthout any plasmide - E. coli Tg1 without pSB1C3 containing the RFP coding sequence - E. coli complemented with our biobrick Bba_K1951011 before and after induction. (you can observe the production of the 4 proteins on the figure below on the left; right : before induction, left : after induction)

Proof of fonctionnality

We investigated if DesA (Lysine decarboxylase) was fonctionnal, by measurement of cadaverine using HPLC with C18 column and proofed our biobrick is a producer of this protein and makes it fonctionnal.

In the futur

We plan to test the whole pathway viability in the first hand. In a second hand, the potential adsorption of platium by our siderophore would be investigate in a rich metal medium.

Biosorption result

Proof of recovered swimming

We have made a biobrick BbaK1951008 ables to produce Flagellin (FliC protein of the flagellum). In the aim to test the flagellin integrity, we made a fliC mutant in a E.coli W3110 strain by transduction using phage P1 (protocol available on our website). fliC mutant has been complemented by our biobrick Bba_K1951008. On the following figure, we investigated if the complemented fliC mutant recovered swimming after complementation. To do this, we did a swimming test using soft gelose. WT W3110 strain was well swimming after 3 hours incubation at 37°C. However, FliC mutant wasn't able to swim whereas fliC mutant complemented recover the swimming ability, making a proof that our biobrick is production a fonctionnal flagellin protein.

Microscopie of the flagellum

We analysed the fliC mutant complemented by BbaK1951008 using electronic microscopy. This tools allowed us to observe the flagellum integrity recovered and to obtain image of our work.

In the futur

We plan to make a proof of concept of the platinum absorption to the surface of the flagellin. In a second time, we want to finish the insertion of the restriction site to allow the cassette insertion ( peptide absorbing specifically precious metal) and test their affinity.

What should this page contain?

Clearly and objectively describe the results of your work.
Future plans for the project
Considerations for replicating the experiments

Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

A list of linked bullet points of the successful results during your project
A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

Inspiration

See how other teams presented their results.

<a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a>
<a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a>
<a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a>

@@ Line 22: / Line 22: @@
 ==Biosorption result==
+===Proof of recovered swimming===
+We have made a biobrick BbaK1951008 ables to produce Flagellin (FliC protein of the flagellum). In the aim to test the flagellin integrity, we made a fliC mutant in a E.coli W3110 strain by transduction using phage P1 (protocol available on our website). fliC mutant has been complemented by our biobrick Bba_K1951008. On the following figure, we investigated if the complemented fliC mutant recovered swimming after complementation. To do this, we did a swimming test using soft gelose. WT W3110 strain was well swimming after 3 hours incubation at 37°C. However, FliC mutant wasn't able to swim whereas fliC mutant complemented recover the swimming ability, making a proof that our biobrick is production a fonctionnal flagellin protein.
 [[File:T--Aix-Marseille--result2.jpeg|1000px|center]]
+===Microscopie of the flagellum===
+We analysed the fliC mutant complemented by BbaK1951008 using electronic microscopy. This tools allowed us to observe the flagellum integrity recovered and to obtain image of our work.
+===In the futur===
+We plan to make a proof of concept of the platinum absorption to the surface of the flagellin.
+In a second time, we want to finish the insertion of the restriction site to allow the cassette insertion ( peptide absorbing specifically precious metal) and test their affinity.
 <div class='content_wrapper'>
 <div class='text_box'>
-<p>Here you can describe the results of your project and your future plans. </p>
 <h5>What should this page contain?</h5>

Difference between revisions of "Team:Aix-Marseille/Results"

Revision as of 14:23, 13 October 2016

Contents

Design

MObilisation result

Proof of fonctionnality

In the futur

Biosorption result

Proof of recovered swimming

Microscopie of the flagellum

In the futur

What should this page contain?

Project Achievements

Inspiration