(→Part Composition:) |
(→Part Composition:) |
||
| Line 7: | Line 7: | ||
* Bba_B0034 : pARA/araC inductible promoter | * Bba_B0034 : pARA/araC inductible promoter | ||
* Bba_K1951000 : Lysine decarboxylase | * Bba_K1951000 : Lysine decarboxylase | ||
| − | |||
====Lysine decarboxylase DesA:==== | ====Lysine decarboxylase DesA:==== | ||
This part is composed of 3 subparts :
Lysine descarboxylase from Streptomyces coelicolor is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.
We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for E.coli and ordered the synthesis by addition of an inductible promoter.
This part is a composite part composed of 2 Biobricks :
Flagellin C (FliC) protein from Escherichia coli strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...
We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110
The subparts were assembled using standard BioBrick Assembly.
