Difference between revisions of "Team:Exeter/Model"

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                 <p id="pp">Protein production:</p>
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                 <h6>Protein production:</hr>
  
 
                 <p id="pp">At the outset, multiple assumptions were made to simplify the the system, enabling suitable definition of  appropriate variables and rates. Mutations were also ignored to eradicate this further layer of complexity. Mindful that the timeframe considered was much larger than the replication time of <i>E. coli</i>,  the amount of mRNA, Protein etc was adjusted accordingly by assuming that it splits evenly and halving the amount every time replication occurs, also affecting the amount of plasmids. However,  as cited in (Nordström and Dasgupta, 2006), the frequency of replication is variable to maintain a constant amount. Therefore, in the case of our plasmid pSB1C3, which had a high copy number ~300, the amount was kept constant even after replication of the <i>E. Coli</i>.</p>
 
                 <p id="pp">At the outset, multiple assumptions were made to simplify the the system, enabling suitable definition of  appropriate variables and rates. Mutations were also ignored to eradicate this further layer of complexity. Mindful that the timeframe considered was much larger than the replication time of <i>E. coli</i>,  the amount of mRNA, Protein etc was adjusted accordingly by assuming that it splits evenly and halving the amount every time replication occurs, also affecting the amount of plasmids. However,  as cited in (Nordström and Dasgupta, 2006), the frequency of replication is variable to maintain a constant amount. Therefore, in the case of our plasmid pSB1C3, which had a high copy number ~300, the amount was kept constant even after replication of the <i>E. Coli</i>.</p>

Revision as of 22:31, 13 October 2016