Line 46: | Line 46: | ||
<img src="https://static.igem.org/mediawiki/2016/5/5a/Result1igem.jpg"> | <img src="https://static.igem.org/mediawiki/2016/5/5a/Result1igem.jpg"> | ||
</p> | </p> | ||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> July 22nd:</strong> Too heated, without result. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/9/9b/Result2igem.jpg"> | ||
+ | </p> | ||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> July 22rd:</strong> the sample was too heated again, and we did not obtained result. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/e/e9/Result3igem.jpg"> | ||
+ | </p> | ||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> July 23rd:</strong> performed in a small chamber gel 1.5, with Plux promoter, not able to see the two expected bands. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/1/1d/Result4igem.jpg"> | ||
+ | </p> | ||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> July 30th:</strong> No result was obtained from all samples, it will be repeated. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/1/1e/Result5igem.jpg"> | ||
+ | </p> | ||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> August 5th:</strong> the expected samples could be clearly seen, the third well tends to have two bands. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/d/d6/Result6igem.jpg"> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> August 10th:</strong> plasmids concentrates, all the samples were completely able to be seen, getting the expected result. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/1/1e/Result7igem.jpg"> | ||
+ | </p> | ||
+ | |||
+ | <h2 style=" | ||
+ | color: black; | ||
+ | text-align: center; | ||
+ | padding: 2%; | ||
+ | font-size: 30px; | ||
+ | margin-bottom: 60px; | ||
+ | font-weight: 200; | ||
+ | "><strong> August 12th: </strong>we turned to repeat samples to make sure everything is correct, then the plasmids were sent to cryogenics. | ||
+ | </h2> | ||
+ | |||
+ | <p style="text-align:center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/c/cf/Result8igem.jpg"> | ||
+ | </p> | ||
+ | |||
Revision as of 03:27, 14 October 2016
After carrying out laboratory work, the way we used to check if our work was successful was through electrophoresis using agarose gel 0.8; 1.2 and 1.5 depending on its need.
Our laboratory work began in June this year and since then, we have been working.
Now, we will see our performance in Lux:
Results
July 8rd: our samples failed to be clear.
July 22nd: Too heated, without result.
July 22rd: the sample was too heated again, and we did not obtained result.
July 23rd: performed in a small chamber gel 1.5, with Plux promoter, not able to see the two expected bands.
July 30th: No result was obtained from all samples, it will be repeated.
August 5th: the expected samples could be clearly seen, the third well tends to have two bands.
August 10th: plasmids concentrates, all the samples were completely able to be seen, getting the expected result.
August 12th: we turned to repeat samples to make sure everything is correct, then the plasmids were sent to cryogenics.