Difference between revisions of "Team:ASIJ Tokyo/Parts"

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         <li class="active"><a href="https://2016.igem.org/Team:ASIJ_Tokyo">Home</a></li>
 
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           <a class="dropdown-toggle" data-toggle="dropdown" href="https://2016.igem.org/ASIJProjectDescription">Our Project<span class="caret"></span></a>
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           <a class="dropdown-toggle" data-toggle="dropdown" href="">The Project<span class="caret"></span></a>
 
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             <li><a href="#">Page 1-1</a></li>
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             <li><a href="https://2016.igem.org/ASIJProjectDescription">Project Description + Abstract</a></li>
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             <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Experiments">Experiments</a></li>
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             <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Results">Results</a></li>
 
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         <li><a href="https://2016.igem.org/ASIJJournal">Journal</a></li>
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         <li><a href="https://2016.igem.org/ASIJBIO">Our Team</a></li>
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         <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Team">The Team</a></li>
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         <li><a href="https://2016.igem.org/ASIJParts">Parts</a></li>
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         <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Attributions">Attributions</a></li>
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         <li><a href="https://2016.igem.org/ASIJPROTOCOL">Protocol</a></li>
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         <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Parts">Parts</a></li>
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Revision as of 03:27, 14 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Parts


  1. Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
  2. Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
  3. Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters
  4. N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest
  5. C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion
  6. PETase sequence: Needed the enzyme to degrade PET plastic
  7. Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
  8. High efficiency e. Coli cells: Were the host for our optimized PETase