Difference between revisions of "Team:ASIJ Tokyo/Parts"
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<li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly | <li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly | ||
<li>High efficiency e. Coli cells: Were the host for our optimized PETase | <li>High efficiency e. Coli cells: Were the host for our optimized PETase | ||
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Revision as of 06:37, 14 October 2016
Parts
- Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
- Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
- Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters
- N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest
- C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion
- PETase sequence: Needed the enzyme to degrade PET plastic
- Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
- High efficiency e. Coli cells: Were the host for our optimized PETase
