Difference between revisions of "Team:ASIJ Tokyo/Parts"

Line 8,011: Line 8,011:
 
                   <h1>Parts </h1>
 
                   <h1>Parts </h1>
 
<br>
 
<br>
−
<ul background: #ffffff;>
+
ul {background: #ffffff;}
 
<li> Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
 
<li> Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
 
<li>Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
 
<li>Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
Line 8,020: Line 8,020:
 
<li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
 
<li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
 
<li>High efficiency e. Coli cells: Were the host for our optimized PETase
 
<li>High efficiency e. Coli cells: Were the host for our optimized PETase
−
</li></ul>
+
</li>
 +
</ul>
  
 
                 </div>
 
                 </div>

Revision as of 06:38, 14 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Parts


ul {background: #ffffff;}
  • Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters
  • Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters
  • Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters
  • N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest
  • C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion
  • PETase sequence: Needed the enzyme to degrade PET plastic
  • Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly
  • High efficiency e. Coli cells: Were the host for our optimized PETase