Difference between revisions of "Team:ASIJ Tokyo/Parts"
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<h1>Parts </h1> | <h1>Parts </h1> | ||
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| − | <li> Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters | + | <li> Weak promoter: Wanted a weak promoter to measure its PET plastic degradation up against the other promoters</li> |
| − | <li>Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters | + | <li>Moderate promoter: Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters</li> |
| − | <li>Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters | + | <li>Strong promoter: Wanted a strong promoter to measure its PET plastic degradation up against the other promoters</li> |
| − | <li>N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest | + | <li>N-Osmy tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest</li> |
| − | <li>C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion | + | <li>C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion</li> |
| − | <li>PETase sequence: Needed the enzyme to degrade PET plastic | + | <li>PETase sequence: Needed the enzyme to degrade PET plastic</li> |
| − | <li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly | + | <li>Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly</li> |
| − | <li>High efficiency e. Coli cells: Were the host for our optimized PETase | + | <li>High efficiency e. Coli cells: Were the host for our optimized PETase</li> |
| − | </li> | + | |
Revision as of 06:45, 14 October 2016
