Difference between revisions of "Team:OUC-China/Safety"

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{{OUC-China}}
 
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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<h5>Safe Lab Work</h5>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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<li><a href="#">Safety</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
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<li class="active"><a href="#float01">Safe Project Design</a></li>
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<li><a href="#float02">Safe Lab Work</a></li>
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<li><a href="#float03">Reference</a></li>
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<h3><b>General Support:</b></h3>
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<p>Harmless bacteria, <i>E.coli.K12</i> is utilized as the main chassis for our prior design on the DNA sequence, stem loop. When further applied into the reconstruction of metabolic circuits, stem loop is harmless for the growth of bacteria and relatively safe for other applications. Being responsible for our earth and ourselves is our attitude in every experiments. What’s more, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. All the chassis organisms will be sterilized by Autoclaves Sterilizers before being abandoned to prevent the genes from leaking..</p>
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<hr>
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<br id="float02">
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<h3><b>Safe Lab Work</b></h3>
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<p>We worked at a neat lab named 105, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary demage. </p>
 +
<br />
 +
<p>No experiment carries no risks to the experimenters, hence we are properly equipped with facilities, such as gloves, googles, lab coats and so on to protect us from biotic and abiotic hazards.</p>
 +
<br />
 +
<p>Then for lab safety, we perform a routine inspection twice a day to ensure the normal operation of our lab and away from non-biological hazards, especially the water and electricity. Furthermore, several operational zones are divided scientifically for specially experiments like RNA extraction and qRT-PCR.</p>
 +
<p>One case where we had to take extra safety precautions was when we gel extracted bands from electrophoresis gels. We wore UV protection goggles to protect our eyes from the UV light.</p>
 +
<br />
 +
<p>For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.</p>
 +
<hr>
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<br id="float03">
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<h3><b>Safe Shipment</b></h3>
 +
<p>For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry and exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious  red tape. Fortunately, Genscript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to Genscript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.</p>
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<div class="row">
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<h3 class="text-center" style="margin-top:20px;">Cistrons Concerto</h3>
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</div>
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<div class="row oucInformation">
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<div class="col-md-7">
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<b>About</b>
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<p>Thanks to:<i><img src="image/ouc-footer-1.fw.png"></i><i><img src="image/ouc-footer-2.fw.png"></i></p>
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<p>Designed and built by @ Jasmine Chen and @ Zexin Jiao</p>
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<p>Code licensed under Apache License v2.0</p>
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</div>
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<div class="col-md-5">
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<b>Contact us</b>
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<p>E-mail: oucigem@163.com</p>
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<p>Follow us on Facebook@ iGEM OUC<i><img src="image/ouc-footer-3.fw.png"></i></p>
 +
<p>Find us on Google Map</p>
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<p>We are OUC-iGEM<i><img src="image/ouc-footer-4.fw.png"></i><i><img src="image/ouc-footer-5.fw.png"></i><i><img src="image/ouc-footer-6.fw.png"></i></p>
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Revision as of 10:43, 14 October 2016

Safety


General Support:

Harmless bacteria, E.coli.K12 is utilized as the main chassis for our prior design on the DNA sequence, stem loop. When further applied into the reconstruction of metabolic circuits, stem loop is harmless for the growth of bacteria and relatively safe for other applications. Being responsible for our earth and ourselves is our attitude in every experiments. What’s more, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. All the chassis organisms will be sterilized by Autoclaves Sterilizers before being abandoned to prevent the genes from leaking..


Safe Lab Work

We worked at a neat lab named 105, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary demage.


No experiment carries no risks to the experimenters, hence we are properly equipped with facilities, such as gloves, googles, lab coats and so on to protect us from biotic and abiotic hazards.


Then for lab safety, we perform a routine inspection twice a day to ensure the normal operation of our lab and away from non-biological hazards, especially the water and electricity. Furthermore, several operational zones are divided scientifically for specially experiments like RNA extraction and qRT-PCR.

One case where we had to take extra safety precautions was when we gel extracted bands from electrophoresis gels. We wore UV protection goggles to protect our eyes from the UV light.


For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.


Safe Shipment

For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry and exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, Genscript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to Genscript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.




Cistrons Concerto

About

Thanks to:

Designed and built by @ Jasmine Chen and @ Zexin Jiao

Code licensed under Apache License v2.0

Contact us

E-mail: oucigem@163.com

Follow us on Facebook@ iGEM OUC

Find us on Google Map

We are OUC-iGEM