Difference between revisions of "Team:Paris Saclay/Parts"

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|Part expressing the dimerization protein FRB* and GFP11
 
|Part expressing the dimerization protein FRB* and GFP11
 
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|[http://parts.igem.org/Part:BBa_K2039006/ BBa_K2039006]
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|Part expressing the protein GFP 1.9
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|[http://parts.igem.org/Part:BBa_K2039002/ BBa_K2039002]
 
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|Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
 
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|[http://parts.igem.org/Part:BBa_K2039007/ BBa_K2039007]
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|Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9)
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Revision as of 16:52, 14 October 2016

Parts

Existing parts used for the project

In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:


Name Type Description Designer Length (bp)
[http://parts.igem.org/Part:BBa_J23119/ BBa_J23119] Basic Constitutive promoter family member iGEM2006_Berkeley 35
[http://parts.igem.org/Part:BBa_B0030/ BBa_B0030] Basic RBS.1 (strong) - modified from R. Weiss Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003 15
[http://parts.igem.org/Part:BBa_B0015/ BBa_B0015] Basic Double terminator (B0010-B0012) (rrnB T1 - T7Te) Reshma Shetty, 2003 129

Parts designed for the project

In order to achieve our project; we designed several basic and composite parts:


Name Type Description Length (bp)
[http://parts.igem.org/Part:BBa_K2039003/ BBa_K2039003] Basic Coding sequences of proteins FKBP and GFP10 528
[http://parts.igem.org/Part:BBa_K2039004/ BBa_K2039004] Basic Coding sequences of proteins FRB* and GFP11 498
[http://parts.igem.org/Part:BBa_K2039000/ BBa_K2039000] Composite Part expressing the dimerization protein FKBP and GFP10 731
[http://parts.igem.org/Part:BBa_K2039001/ BBa_K2039001] Composite Part expressing the dimerization protein FRB* and GFP11 701
[http://parts.igem.org/Part:BBa_K2039006/ BBa_K2039006] Composite Part expressing the protein GFP 1.9 836
[http://parts.igem.org/Part:BBa_K2039002/ BBa_K2039002] Composite Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 1440
[http://parts.igem.org/Part:BBa_K2039007/ BBa_K2039007] Composite Dimerization system FRB/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9) 2284





Université Paris Sud
91405 Orsay, France
Copyright © 2016 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.