Difference between revisions of "Team:UofC Calgary/sandbox"

 
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{{Team:UofC_Calgary/assets/plugins/jquery-migrate.min.js}}
 
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{{Team:UofC_Calgary/assets/plugins/cubeportfolio/js/jquery.cubeportfolio.min.js}}
 
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<header class="c-layout-header c-layout-header-fixed c-layout-header-3 c-layout-header-default-mobile c-bg-darkblue" style="padding-top: 5px;">
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<header class="c-layout-header c-layout-header-3 c-layout-header-default-mobile c-header-seethrough" style="padding-top: 10px;
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<!-- BEGIN: MEGA MENU -->
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<ul class="nav navbar-nav c-theme-nav">
 
<li class="c-active c-menu-type-classic">
 
<li class="c-active c-menu-type-classic">
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<a href="#" class="c-link dropdown-toggle">Home</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/sandbox" class="c-link dropdown-toggle">Home</a>
 
</li>
 
</li>
 
<li class="c-menu-type-classic">
 
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<ul class="dropdown-menu c-menu-type-inline c-pull-left">
 
<li>
 
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<a href="Problem">Problem</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/project/problem/sandbox">Problem</a>
 
</li>
 
</li>
 
<li>
 
<li>
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<a href="Solution">Our Solution</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/project/solution/sandbox">Our Solution</a>
 
</li>
 
</li>
 
                                                                 <li>
 
                                                                 <li>
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<a href="Results">Our Results</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/project/results/sandbox">Our Results</a>
 
</li>
 
</li>
 
                                                                 <li>
 
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<a href="Model">Model</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/project/model/sandbox">Model</a>
 
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<a href="Parts">iGEM Parts</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/project/parts/sandbox">Parts Registry</a>
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</li>
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                                                                <li>
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                                                                <a href="https://2016.igem.org/Team:UofC_Calgary/Composite_Part">Composite Parts</a>
 
</li>
 
</li>
 
</ul>
 
</ul>
 
</li>
 
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<li class="c-menu-type-classic">
 
<li class="c-menu-type-classic">
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<a href="https://2016.igem.org/Team:UofC_Calgary/judging/sandbox" class="c-link dropdown-toggle">Judging</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/judging/sandbox" class="c-link">Judging</a>
 
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<li class="c-menu-type-classic">
 
<a href="https://2016.igem.org/Team:UofC_Calgary/human-practices/sandbox" class="c-link dropdown-toggle">Human Practices</a>
 
<a href="https://2016.igem.org/Team:UofC_Calgary/human-practices/sandbox" class="c-link dropdown-toggle">Human Practices</a>
 
<ul class="dropdown-menu c-menu-type-inline c-pull-left">
 
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<a href="https://2016.igem.org/Team:UofC_Calgary/Outreach/sandbox">Outreach</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/Engagement/sandbox">Engagement</a>
 
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<a href="https://2016.igem.org/Team:UofC_Calgary/notebook/sandbox" class="c-link dropdown-toggle">Notebook</a>
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<a href="https://2016.igem.org/Team:UofC_Calgary/notebook/sandbox" class="c-link">Notebook</a>
 
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<a href="https://2016.igem.org/Team:UofC_Calgary/team/sandbox">Meet Our Team</a>
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<a href="Team">Meet Our Team</a>
 
</li>
 
</li>
 
<li>
 
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             <img alt="" src="https://static.igem.org/mediawiki/2016/7/78/T--UofC_Calgary--bgmin.jpg" data-bgposition="center center" data-bgfit="cover" data-bgrepeat="no-repeat" style="width: 100%; height: 100%;">
 
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                         <div class="c-content-title-1">
 
                         <div class="c-content-title-1">
 
                             <h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
 
                             <h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
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                                         <li class="wow animate fadeInUp" style="visibility: visible; animation-name: fadeInUp; width: 100%;" style="color:#EBDEBE">
 
                                             <h4 style="color:#EBDEBE">Project Description</h4>
 
                                             <h4 style="color:#EBDEBE">Project Description</h4>
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                                            <p>Each year on Earth, individuals receive 2.4 mSv of ionizing radiation (IR), which is easily tolerated by our cells. In space however, without the protection of the magnetosphere, astronauts are exposed to high levels of IR in the range of 50 to 2000 mSv which causes the accumulation of deleterious double strand breaks in DNA. Despite current research into methods of IR protection, many solutions such as radiation shield coating are expensive. Biological solutions such as the use of radioprotectors are also subjected to the sinusoidal pharmacokinetic problem resulting in the need for constant administration and the accumulation of waste. </p> <p>
+
                                            <p style="color: #FFFFFF">Mars and the cosmos are tantalizingly close for Mankind to reach in the next century; however, there are several glaring problems that we must hurdle to reach this objective. Stellar radiation is chief among them. Each year on Earth, individuals receive 2.4 mSv of ionizing radiation (IR), which is easily tolerated by our cells. In space without the protection of the magnetosphere, astronauts are exposed to high levels of IR in the range of 50 to 2000 mSv which causes the accumulation of deleterious double strand breaks in DNA. Despite current research into methods of IR protection, many solutions such as radiation shield coating are expensive especially to transport to space. </p> <p style="color: #FFFFFF">
−
 
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Certain naturally-occuring proteins and peptides, such as the modified Bowman-Birk Inhibitor (mBBI), have been found to confer protection against DNA damage in cells exposed to ionizing radiation. Previous studies have shown that BBI increases the survival rate of cells significantly when irradiated, by augmenting endogenous DNA repair mechanisms. </p><p>
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To solve the problem of creating a cost effective, continuous delivery system for IR protection, we have designed a transdermal patch for the delivery of mBBI through the skin. The patch hosts recombinant <i>Bacillus subtilis</i> that expresses a mBBI gene tagged with a transdermal tag that allows the peptide to travel through the skin layers, and into the bloodstream for dispersal throughout the body. Using <i>B. subtilis</i> for the production of mBBI allows for constant delivery bypassing the sinusoidal pharmacokinetic problem. Its long term, continuous delivery will also create a cost effective solution. Ultimately our transdermal delivery system allows for the administration biotherapeutics within an efficient and practical system.
+
  
 +
Biological solutions such as the use of radioprotectors are also subjected to the sinusoidal pharmacokinetic problem resulting in the need for constant administration and the accumulation of waste. Certain naturally-occurring proteins and peptides, such as the modified Bowman-Birk Inhibitor (mBBI), have been found to confer protection against DNA damage in cells exposed to ionizing radiation. Previous studies have shown that BBI increases the survival rate of cells significantly when irradiated, by augmenting endogenous DNA repair mechanisms.
 +
</p><p style="color: #FFFFFF">
  
−
</p>
+
To solve the problem of creating a cost effective and continuous delivery system for IR protection, we have designed a transdermal patch for the delivery of mBBI through the skin. The patch hosts recombinant <i>Bacillus subtilis</i> that expresses a mBBI gene tagged with a transdermal tag that allows the peptide to travel through the skin layers, and into the bloodstream for dispersal throughout the body. The recombinant protein is also tagged with a secretory tag, which is cleaved off using endogenous proteases before delivery out to the skin. Using B. subtilis for the production of mBBI allows for constant delivery bypassing the sinusoidal pharmacokinetic problem. Its long term, continuous delivery will also create a cost effective solution. Ultimately our transdermal delivery system allows for the administration biotherapeutics within an efficient and practical system, while still maintaining the flexibility of modularity.
 +
</p>  
 
                                 </div>
 
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                             <h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
 
                             <h3 class="c-center c-font-uppercase c-font-bold" style="color:#EBDEBE">Our Project</h3>
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                                             <h4 style="color:#EBDEBE">Radiation</h4>
 
                                             <h4 style="color:#EBDEBE">Radiation</h4>
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                                             <p>Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
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                                             <p style="color:#FFFFFF">Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
 
                                         </li>
 
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                                             <h4 style="color:#EBDEBE">Access to Nutrients</h4>
 
                                             <h4 style="color:#EBDEBE">Access to Nutrients</h4>
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                                             <p style="color:#FFFFFF">Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
 
                                         </li>
 
                                         </li>
 
                                     </ul>
 
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                                             <h4 style="color:#EBDEBE">Resource Limitations</h4>
 
                                             <h4 style="color:#EBDEBE">Resource Limitations</h4>
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                                             <p>Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
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                                             <p style="color:#FFFFFF">Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
 
                                         </li>
 
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                                             <h4 style="color:#EBDEBE">Reusable Framework</h4>
 
                                             <h4 style="color:#EBDEBE">Reusable Framework</h4>
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                                             <p>Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
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                                             <p style="color:#FFFFFF">Lorem ipsum dolor sit amet consectetuer adipiscing elit sed diam nonummy nibh euismod</p>
 
                                         </li>
 
                                         </li>
 
                                     </ul>
 
                                     </ul>
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<h3 class="c-font-uppercase c-font-bold"><i>Escherichia coli</i></h3>
 
<h3 class="c-font-uppercase c-font-bold"><i>Escherichia coli</i></h3>
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                                                                                                         <div class="col-md-4 col-xs-6 c-logo c-logo-1">
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<a href="#"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/3/35/T--UofC_Calgary--uofc.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="http://www.ucalgary.ca/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/3/35/T--UofC_Calgary--uofc.png" alt="" style="width: 180px; height:140px;"></a>
 
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<a href="#"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/74/T--UofC_Calgary--O'BrienLogo.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="http://cumming.ucalgary.ca/bhsc/program/o%E2%80%99brien-centre-facility"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/74/T--UofC_Calgary--O'BrienLogo.png" alt="" style="width: 180px; height:140px;"></a>
 
</div>
 
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<div class="col-md-4 col-xs-6 c-logo c-logo-3">
 
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<a href="#"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/c/c7/T--UofC_Calgary--uofcschulich.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="http://schulich.ucalgary.ca/"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/c/c7/T--UofC_Calgary--uofcschulich.png" alt="" style="width: 180px; height:140px;"></a>
 
</div>
 
</div>
 
<div class="col-md-4 col-xs-6 c-logo c-logo-4">
 
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<a href="#"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/1/1f/T--UofC_Calgary--mindfuel.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="https://www.mindfuel.ca/?gclid=CJ_GjpW0384CFZSIfgodOW4F0A"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/1/1f/T--UofC_Calgary--mindfuel.png" alt="" style="width: 180px; height:140px;"></a>
 
</div>
 
</div>
 
<div class="col-md-4 col-xs-6 c-logo c-logo-5">
 
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<a href="#"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/7d/T--UofC_Calgary--geekstarter.png" alt="" style="width: 180px; height:140px;"></a>
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<a href="http://www.albertatechfutures.ca/Research/BasicResearch/geekStarter.aspx"><img class="c-img-pos" src="https://static.igem.org/mediawiki/2016/7/7d/T--UofC_Calgary--geekstarter.png" alt="" style="width: 180px; height:140px;"></a>
 
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<div class="col-md-4 col-xs-6 c-logo c-logo-6">
 
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Latest revision as of 19:17, 14 October 2016

iGEM Calgary 2016

Our Project

  • Project Description

    Mars and the cosmos are tantalizingly close for Mankind to reach in the next century; however, there are several glaring problems that we must hurdle to reach this objective. Stellar radiation is chief among them. Each year on Earth, individuals receive 2.4 mSv of ionizing radiation (IR), which is easily tolerated by our cells. In space without the protection of the magnetosphere, astronauts are exposed to high levels of IR in the range of 50 to 2000 mSv which causes the accumulation of deleterious double strand breaks in DNA. Despite current research into methods of IR protection, many solutions such as radiation shield coating are expensive especially to transport to space.

    Biological solutions such as the use of radioprotectors are also subjected to the sinusoidal pharmacokinetic problem resulting in the need for constant administration and the accumulation of waste. Certain naturally-occurring proteins and peptides, such as the modified Bowman-Birk Inhibitor (mBBI), have been found to confer protection against DNA damage in cells exposed to ionizing radiation. Previous studies have shown that BBI increases the survival rate of cells significantly when irradiated, by augmenting endogenous DNA repair mechanisms.

    To solve the problem of creating a cost effective and continuous delivery system for IR protection, we have designed a transdermal patch for the delivery of mBBI through the skin. The patch hosts recombinant Bacillus subtilis that expresses a mBBI gene tagged with a transdermal tag that allows the peptide to travel through the skin layers, and into the bloodstream for dispersal throughout the body. The recombinant protein is also tagged with a secretory tag, which is cleaved off using endogenous proteases before delivery out to the skin. Using B. subtilis for the production of mBBI allows for constant delivery bypassing the sinusoidal pharmacokinetic problem. Its long term, continuous delivery will also create a cost effective solution. Ultimately our transdermal delivery system allows for the administration biotherapeutics within an efficient and practical system, while still maintaining the flexibility of modularity.

Our Project

  • Radiation

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  • Access to Nutrients

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  • Resource Limitations

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  • Reusable Framework

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Escherichia coli

Escherichia coli is a gram negative bacteria that is one of the key bacteria used by our team this year. We are working with E. coli to test our ideas before moving to more difficult to work with Bacillus subtilis.

Bacillus subtilis

Bacillus subtilis is the gram-positive version of E. coli in the world of synthetic biology. B. subtilis is the workhorse bacteria for our project, living in the patch and secreting the BBI.

Deinococcus radiodurans

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Our Project

Click to learn more about each part of our project

About us

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  • Lit af project
  • Sick lab
  • Huge fam

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Our Sponsors

iGEM

iGEM is an international competition promoting synthetic biology as a means to solve social, economic and humanitarian problems around the globe. The iGEM Jamboree is held in Boston annually. In 2016, over 300 teams are competing against each other.

Latest Entries

BBI & KTI Have Arrived!

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Fully Trained!

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Read More

Latest Pictures

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Find us

Located in Calgary, Alberta, Canada.

  • University of Calgary
  • +587 717 7233
  • syed.jafri2@ucalgary.ca

Copyright © University of Calgary 2016.