Difference between revisions of "Team:CU-Boulder/Composite Part"

Revision as of 03:47, 15 October 2016

Composite Part

G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.

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-<h3> Basic Part </h3>
+<h3> Composite Part </h3>
-<p class = "main"> EutS(BBa_K2129001) is derived from EutSMNLK (BBa_K31100). This single protein is capable of forming bacterial microcompartments on it’s own, and can be visualized with EutC 1-19 tagged fluorescent proteins. We think that this part presents exciting opportunities for future teams; the EutC tag enables any small enough protein to be isolated within these cages, which has an enormous range of potential applications.</p>
+<p class = "main"> G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.</p>
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