Line 9: | Line 9: | ||
<br><br><br> | <br><br><br> | ||
<p>In order to design short hairpin RNA (shRNA) conform to demands in our experiments, we cooperated with SYSU-Software from Sun Yat-sen University to develop a corresponding software. We provided algorithms and design requirements, and SYSU-Software wrote this delicate software for us. With its help, we successfully designed K-ras siRNA, whose sequence and predicted structure are shown below. We validated that this shRNA was able to down-regulate the expression level of K-ras in A549 effectively. We thank our collaborator for receiving us with attention and for such strong contribution to our work</p> | <p>In order to design short hairpin RNA (shRNA) conform to demands in our experiments, we cooperated with SYSU-Software from Sun Yat-sen University to develop a corresponding software. We provided algorithms and design requirements, and SYSU-Software wrote this delicate software for us. With its help, we successfully designed K-ras siRNA, whose sequence and predicted structure are shown below. We validated that this shRNA was able to down-regulate the expression level of K-ras in A549 effectively. We thank our collaborator for receiving us with attention and for such strong contribution to our work</p> | ||
− | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/e/ea/NJU_China_2016_iGEM_collaborations.jpg"> |
</body> | </body> | ||
</html> | </html> |
Revision as of 05:31, 15 October 2016
In order to design short hairpin RNA (shRNA) conform to demands in our experiments, we cooperated with SYSU-Software from Sun Yat-sen University to develop a corresponding software. We provided algorithms and design requirements, and SYSU-Software wrote this delicate software for us. With its help, we successfully designed K-ras siRNA, whose sequence and predicted structure are shown below. We validated that this shRNA was able to down-regulate the expression level of K-ras in A549 effectively. We thank our collaborator for receiving us with attention and for such strong contribution to our work