Difference between revisions of "Team:Northwestern/08 18"

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   <article>
 
   <article>
 
     <h1>Thursday, August 18<sup>th</sup></h3>
 
     <h1>Thursday, August 18<sup>th</sup></h3>
−
     <h2>Agenda:</h2>
+
     <h2>Tasks:</h2>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Jordan</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Ran RTW PCR of Cas9 for SS with Sara
 +
            <div class="row">
 +
              <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/c/c9/T--Northwestern--08_18_1.png" width="1600" height="1200" alt=""/></div>
 +
            </div>
 +
          </li>
 +
          <li>Inoculated overnight cultures of JC8031 and JC8031-pClyA-GFP-His6 </li>
 +
          <li>Gel extracted linearized Cas9 for signal sequences </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul style="">
 +
          <li>Ran gel for linearized Cas9 and linearized mRFP
 +
<div class="row">
 +
              <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/f/f6/T--Northwestern--08_18_2.png" width="2448" height="3264" alt=""/></div>
 +
            </div>
 +
</li>
 +
          <li>Ran gel for 8/16/16 gibson reaction products
 +
           
 +
            <div class="row">
 +
              <div class="col-sm-6"><img src="https://static.igem.org/mediawiki/2016/5/52/T--Northwestern--08_18_3.jpg" width="709" height="716" alt=""/></div>
 +
            </div>
 +
          </li>
 +
          <li>1: 2kb Ladder</li>
 +
          <li>3: Cas9 Gibson with TorA signal (expected size: ~5kb; parts: ~5k, ~300bp)</li>
 +
          <li>5: Cas9 Gibson with INP fusion (expected size: ~6kb; parts: ~5kb, ~1kb)</li>
 +
          <li>7: mRFP Gibson with gRNA (expected size: ~4kb; parts: ~3.5kb, 400bp)</li>
 +
          <li>9: Gibson kit positive control</li>
 +
          <li>10: No Gibson mix negative control w/ Cas9 and TorA (expected sizes: ~5kb, ~300bp)</li>
 +
          <li> </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on presentation for profs </li>
 +
          <li>Helped set up GFP/mCherry PCR (Thush did it)</li>
 +
          <li>Troubleshoot Gibson with Kelly</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Finally put research in the research doc</li>
 +
          <li>Looked over presentation for tomorrow</li>
 +
          <li>Started designing antibiotics forum</li>
 +
        </ul>
 +
      </div>
 +
    </div>
  
 
</article>
 
</article>

Revision as of 22:00, 15 October 2016

Notebook

Thursday, August 18th

Tasks:

Jordan

  • Ran RTW PCR of Cas9 for SS with Sara
  • Inoculated overnight cultures of JC8031 and JC8031-pClyA-GFP-His6
  • Gel extracted linearized Cas9 for signal sequences

Michelle

  • Ran gel for linearized Cas9 and linearized mRFP
  • Ran gel for 8/16/16 gibson reaction products
  • 1: 2kb Ladder
  • 3: Cas9 Gibson with TorA signal (expected size: ~5kb; parts: ~5k, ~300bp)
  • 5: Cas9 Gibson with INP fusion (expected size: ~6kb; parts: ~5kb, ~1kb)
  • 7: mRFP Gibson with gRNA (expected size: ~4kb; parts: ~3.5kb, 400bp)
  • 9: Gibson kit positive control
  • 10: No Gibson mix negative control w/ Cas9 and TorA (expected sizes: ~5kb, ~300bp)

Paul

  • Worked on presentation for profs
  • Helped set up GFP/mCherry PCR (Thush did it)
  • Troubleshoot Gibson with Kelly

Sam

  • Finally put research in the research doc
  • Looked over presentation for tomorrow
  • Started designing antibiotics forum