Difference between revisions of "Team:BGIC China"
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<h2> Project Description </h2> | <h2> Project Description </h2> | ||
| − | <p>As the first high school team of BGI-college, we endeavour to apply the cell-free system to a semi-quantitative test paper with the reaction site in the shape of a pearl-necklace whose results can be observed by human to measure the concentration of target molecules in the sample within a certain range. This mechanism integrated with biosensors for target molecules enabled the testing of the concentration of hormones, glucose, fatty acid or nitrates in clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience. | + | <p>As the first high school team of BGI-college, we endeavour to apply the cell-free system to a semi-quantitative test paper with the reaction site in the shape of a pearl-necklace whose results can be observed by human to measure the concentration of target molecules in the sample within a certain range. This mechanism integrated with biosensors for target molecules enabled the testing of the concentration of hormones, glucose, fatty acid or nitrates in clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience.</p> |
| − | + | <p>Our idea originated from comprehensive discussions that eliminated the alternatives of acetochlor test papers which already exists in the market at a feasible price and the cholinesterase injection plan for protection of nerve system against Sarin gas as the project relied upon the injected medicine’s access through the Brain-Blood Barriers——an unsolvable dilemma. We procured research that followed which can be traced all the way back to November 2015. It was by chance that we came across the article Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates written by Alexis Courbet, Drew Endy, Eric Renard, Franck Molina, and Jérôme Bonnet along with Keith Pardee’s articles on paper-based synthetic gene networks. The two articles outlined the feasibility of a paper-based cell-free system for inspection of clinical samples targeting at specific molecules as we grappled what they meant to us, and became the very foundation of our project. In late April, the designing of our plasmids was initiated via comprehensive methods, which, upon finishing even as we post this passage, has gone through three phases. Upon the biosensor’s contact with the target molecule, the genetic circuit will produce an output with the fluorescence of a GFP.</p> | |
| − | + | <p>Our goals go as follows. The very foundation of our targets is to apply our cell-free system, the system of cell environment simulation which consists of cell-extract and substrate that enables DNA expression in vitro to a mature test paper module. Laboratory sample and clinical sample tests will be carried out to assess the effectiveness of the coalition. To make that happen, we will test the feasibility of our test paper prototype with pearl necklace-shaped reaction region and enhance its operability through modelling until a semi-quantitative test-paper system is built up. Should that succeed, we will expand the project into the designing, testing and, if possible, manufacturing of a test paper reader: To assemble a test paper reader according to existing blueprints and express the measurements via reporter proteins from single apertures. </p> | |
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Revision as of 07:03, 30 June 2016
Welcome to iGEM 2016!
Your team has been approved and you are ready to start the iGEM season!
Project Description
As the first high school team of BGI-college, we endeavour to apply the cell-free system to a semi-quantitative test paper with the reaction site in the shape of a pearl-necklace whose results can be observed by human to measure the concentration of target molecules in the sample within a certain range. This mechanism integrated with biosensors for target molecules enabled the testing of the concentration of hormones, glucose, fatty acid or nitrates in clinical samples of plasma, urine or saliva. Quantitative results can be shown by the output of a variety of proteins.(light emission of GFP or the changing of colour initiated by LacZ,etc.). The high portability of the test paper and the small duration of the testing process of clinical samples, should the test paper be successfully manufactured, shall relieve millions of patients of comprehensive time-consuming and perhaps agonising medical inspections—these could be carried out at home! The test paper system, presumably, transcends mainstream medical inspections of clinical samples in means of efficiency and convenience.
Our idea originated from comprehensive discussions that eliminated the alternatives of acetochlor test papers which already exists in the market at a feasible price and the cholinesterase injection plan for protection of nerve system against Sarin gas as the project relied upon the injected medicine’s access through the Brain-Blood Barriers——an unsolvable dilemma. We procured research that followed which can be traced all the way back to November 2015. It was by chance that we came across the article Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates written by Alexis Courbet, Drew Endy, Eric Renard, Franck Molina, and Jérôme Bonnet along with Keith Pardee’s articles on paper-based synthetic gene networks. The two articles outlined the feasibility of a paper-based cell-free system for inspection of clinical samples targeting at specific molecules as we grappled what they meant to us, and became the very foundation of our project. In late April, the designing of our plasmids was initiated via comprehensive methods, which, upon finishing even as we post this passage, has gone through three phases. Upon the biosensor’s contact with the target molecule, the genetic circuit will produce an output with the fluorescence of a GFP.
Our goals go as follows. The very foundation of our targets is to apply our cell-free system, the system of cell environment simulation which consists of cell-extract and substrate that enables DNA expression in vitro to a mature test paper module. Laboratory sample and clinical sample tests will be carried out to assess the effectiveness of the coalition. To make that happen, we will test the feasibility of our test paper prototype with pearl necklace-shaped reaction region and enhance its operability through modelling until a semi-quantitative test-paper system is built up. Should that succeed, we will expand the project into the designing, testing and, if possible, manufacturing of a test paper reader: To assemble a test paper reader according to existing blueprints and express the measurements via reporter proteins from single apertures.
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Tips
This wiki will be your team��s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don��t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
